A cell line with decreased sensitivity to the methyl mercury-induced stimulation of α-amanitin sensitive RNA synthesis in isolated nuclei

Gerald D. Frenkel, Janet Ducote, Christian P. Reboulleau, John Gierthy

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2 Scopus citations

Abstract

1. 1. In nuclei isolated from cells of the B50 rat neuroblastoma line the stimulatory effect of methyl mercury on α-amanitin-sensitive RNA synthesis is very much reduced compared to the stimulatory effect in HeLa nuclei (see: Frenkel G. D. and Randles K. (1982) Specific stimulation of α-amanitin-sensitive RNA synthesis in isolated HeLa nuclei by methyl mercury. J. biol. Chem. 257, 6275-6279). 2. 2. The stimulatory effect of another mercury compound, p-hydroxymercuribenzoate, was also much less pronounced in the B50 nuclei. 3. 3. Similar results were obtained with nuclei isolated from B50 cells which had been induced to differentiate by exposure to dibutaryl cyclic AMP. 4. 4. Nuclei isolated from cells of another rat neuroblastoma line (B35), and nuclei from cells of a human neuroblastoma line both exhibited levels of stimulation similar to that of HeLa nuclei. 5. 5. The B50 and HeLa cells were also compared as to their sensitivity to other effects of methyl mercury.

Original languageEnglish (US)
Pages (from-to)477-482
Number of pages6
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume91
Issue number3
DOIs
StatePublished - 1988

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology

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