A chimeric embryoid body model to study vascular morphogenesis

Yanmei Qi, Siavash Saadat, Jie Liu, Alan M. Graham, Shaohua Li

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Embryonic stem cell (ESC)-derived embryoid body (EB) is a unique model for studying vascular development, in that it provides a three-dimensional microenvironment that mimics an in vivo milieu. When using genetargeting EBs to study certain defects in vascular morphogenesis, it is necessary to determine whether the defect is due to the intrinsic loss of the gene in endothelial cells (EC) or rather due to the lack of surrounding factors that would typically promote vascular development. Here we describe a chimeric EB vessel development model, in which the utilization of the PECAM-GFP reporter gene in wild-type ESCs allows for the introduction of “normal” extracellular factors formed by its parallel differentiation to the gene-deletion EC that might otherwise be devoid of these factors.

Original languageEnglish (US)
Pages (from-to)215-224
Number of pages10
JournalMethods in Molecular Biology
Volume1214
DOIs
StatePublished - Jan 1 2015

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • Cdc42
  • Chimeric EB
  • Embryoid body
  • Embryonic stem cell
  • Endothelial cell
  • PECAM-EGFP
  • Vascular morphogenesis

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