A circadian clock of drosophila: Effects of deuterium oxide and mutations at the period locus

Lori White; John Ringo; Harold Dowse

doi:10.3109/07420529209064534

A circadian clock of drosophila: Effects of deuterium oxide and mutations at the period locus

Lori White, John Ringo, Harold Dowse

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Mutations at the period (per) locus (1:1.3; 3B1-2) in Drosophila melanogaster lengthen (per^L), shorten (per⁵), or abolish (per° overt circadian rhythmi-city. Deuterium oxide lengthens the free-running circadian period. We tested the effects of deuterium on three mutants of the per gene (per⁵ per^L, and per° and wild-type Drosophila melanogaster (per⁺) to assess interactions. With increasing concentrations of deuterium, the free-running circadian period of locomotor activity rhythms increased. The dose-response was linear in all genotypes tested. With increasing dosages ofdeuterium, circadian rhythms became weaker as evidenced by the signal-to-noise ratio (SNR). Genotype and deuterium changed circadian period length independently and additively, showing no interaction. SNRs for all genotypes converged on a low level as deuterium concentration increased. Deuterium increased life span, except at high concentrations (40 and 50%.

Original language	English (US)
Pages (from-to)	250-259
Number of pages	10
Journal	Chronobiology International
Volume	9
Issue number	4
DOIs	https://doi.org/10.3109/07420529209064534
State	Published - 1992
Externally published	Yes

All Science Journal Classification (ASJC) codes

Physiology
Physiology (medical)

Keywords

Deuterium oxide
Drosophila
Life span
Period mutations

Access to Document

10.3109/07420529209064534

Cite this

@article{1884bd8b85e54eebb7c668f933908f5d,

title = "A circadian clock of drosophila: Effects of deuterium oxide and mutations at the period locus",

abstract = "Mutations at the period (per) locus (1:1.3; 3B1-2) in Drosophila melanogaster lengthen (perL), shorten (per5), or abolish (per° overt circadian rhythmi-city. Deuterium oxide lengthens the free-running circadian period. We tested the effects of deuterium on three mutants of the per gene (per5 perL, and per° and wild-type Drosophila melanogaster (per+) to assess interactions. With increasing concentrations of deuterium, the free-running circadian period of locomotor activity rhythms increased. The dose-response was linear in all genotypes tested. With increasing dosages ofdeuterium, circadian rhythms became weaker as evidenced by the signal-to-noise ratio (SNR). Genotype and deuterium changed circadian period length independently and additively, showing no interaction. SNRs for all genotypes converged on a low level as deuterium concentration increased. Deuterium increased life span, except at high concentrations (40 and 50%.",

keywords = "Deuterium oxide, Drosophila, Life span, Period mutations",

author = "Lori White and John Ringo and Harold Dowse",

year = "1992",

doi = "10.3109/07420529209064534",

language = "English (US)",

volume = "9",

pages = "250--259",

journal = "Chronobiology International",

issn = "0742-0528",

publisher = "Taylor and Francis Ltd.",

number = "4",

}

TY - JOUR

T1 - A circadian clock of drosophila

T2 - Effects of deuterium oxide and mutations at the period locus

AU - White, Lori

AU - Ringo, John

AU - Dowse, Harold

PY - 1992

Y1 - 1992

N2 - Mutations at the period (per) locus (1:1.3; 3B1-2) in Drosophila melanogaster lengthen (perL), shorten (per5), or abolish (per° overt circadian rhythmi-city. Deuterium oxide lengthens the free-running circadian period. We tested the effects of deuterium on three mutants of the per gene (per5 perL, and per° and wild-type Drosophila melanogaster (per+) to assess interactions. With increasing concentrations of deuterium, the free-running circadian period of locomotor activity rhythms increased. The dose-response was linear in all genotypes tested. With increasing dosages ofdeuterium, circadian rhythms became weaker as evidenced by the signal-to-noise ratio (SNR). Genotype and deuterium changed circadian period length independently and additively, showing no interaction. SNRs for all genotypes converged on a low level as deuterium concentration increased. Deuterium increased life span, except at high concentrations (40 and 50%.

AB - Mutations at the period (per) locus (1:1.3; 3B1-2) in Drosophila melanogaster lengthen (perL), shorten (per5), or abolish (per° overt circadian rhythmi-city. Deuterium oxide lengthens the free-running circadian period. We tested the effects of deuterium on three mutants of the per gene (per5 perL, and per° and wild-type Drosophila melanogaster (per+) to assess interactions. With increasing concentrations of deuterium, the free-running circadian period of locomotor activity rhythms increased. The dose-response was linear in all genotypes tested. With increasing dosages ofdeuterium, circadian rhythms became weaker as evidenced by the signal-to-noise ratio (SNR). Genotype and deuterium changed circadian period length independently and additively, showing no interaction. SNRs for all genotypes converged on a low level as deuterium concentration increased. Deuterium increased life span, except at high concentrations (40 and 50%.

KW - Deuterium oxide

KW - Drosophila

KW - Life span

KW - Period mutations

UR - http://www.scopus.com/inward/record.url?scp=0026908176&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026908176&partnerID=8YFLogxK

U2 - 10.3109/07420529209064534

DO - 10.3109/07420529209064534

M3 - Article

C2 - 1330336

AN - SCOPUS:0026908176

SN - 0742-0528

VL - 9

SP - 250

EP - 259

JO - Chronobiology International

JF - Chronobiology International

IS - 4

ER -

A circadian clock of drosophila: Effects of deuterium oxide and mutations at the period locus

Abstract

All Science Journal Classification (ASJC) codes

Keywords

Access to Document

Other files and links

Fingerprint

Cite this