@article{7f07965f8f4b485ea22086236f290e73,
title = "A conserved HEAT domain within eIF4G Directs Assembly of the Translation Initiation Machinery",
abstract = "The X-ray structure of the phylogenetically conserved middle portion of human eukaryotic initiation factor (eIF) 4GII has been determined at 2.4 {\AA} resolution, revealing a crescent-shaped domain consisting of ten α helices arranged as five HEAT repeats. Together with the ATP-dependent RNA helicase eIF4A, this HEAT domain suffices for 48S ribosomal complex formation with a picornaviral RNA internal ribosome entry site (IRES). Structure-based site-directed mutagenesis was used to identify two adjacent features on the surface of this essential component of the translation initiation machinery that, respectively, bind eIF4A and a picornaviral IRES. The structural and biochemical results provide mechanistic insights into both cap-dependent and cap-independent translation initiation.",
author = "Joseph Marcotrigiano and Lomakin, {Ivan B.} and Nahum Sonenberg and Pestova, {Tatyana V.} and Hellen, {Christopher U.T.} and Burley, {Stephen K.}",
note = "Funding Information: We thank Drs. L. Berman, J.B. Bonanno, H.A. Lewis, and K. Musunuru for invaluable assistance with X-ray measurements, and Drs. R.C. Deo, A.-C. Gingras, D. Jeruzalmi, V. Kolupaeva, J. Kuriyan, G.A. Petsko, and M. Romanowski for many useful discussions. We thank Ms. T.B. Niven for editorial assistance. S. K. B. is an Investigator for the Howard Hughes Medical Institute, and N.S. is a Howard Hughes Medical Institute International Scholar. This work was supported by Human Frontiers Scientific Program grant RG0303 (S. K. B., N. S., and Edward Darzynkiewicz); grant GM61262 from the National Institutes of Health (S. K. B.); and grant MCB-9726958 from the National Science Foundation (C. U. T. H.). J. M. was supported by a Burroughs-Wellcome Fund Interfaces Program graduate fellowship and a David Rockefeller graduate fellowship. ",
year = "2001",
doi = "10.1016/S1097-2765(01)00167-8",
language = "English (US)",
volume = "7",
pages = "193--203",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "1",
}