@inproceedings{9c8affa573be4778ae02fdeaa54bf5b0,
title = "A continuous flow, electrically triggered microelectroporator",
abstract = "We present a micro-electroporation device that automatically detects continuously flowing single cells, applies electroporation pulses and monitors cell electrical responses. A phase sensitive sensor scans for the cell membrane impedance signal following each pulse. Key parameters for successful cell membrane permeabilization detection in a flow environment were determined using electrical and computational tools. By varying the electric field parameters, we demonstrate modulation of cell membrane permeabilization by electrically measuring the electroporation-induced cell membrane impedance change and by optically measuring the delivery of a fluorescent probe. Viability of the electroporated cells following collection demonstrates a correlation with the applied pulse strength.",
keywords = "Automation, Electroporation, Microfluidics, Permeabilization, Single cell",
author = "Mingde Zheng and Sherba, {Joseph J.} and Shan, {Jerry W.} and Hao Lin and Shreiber, {David I.} and Zahn, {Jeffrey D.}",
year = "2016",
language = "English (US)",
series = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",
publisher = "Chemical and Biological Microsystems Society",
pages = "1218--1219",
booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",
note = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 ; Conference date: 09-10-2016 Through 13-10-2016",
}