A CUGGU/UUGGU-specific MazF homologue from Methanohalobium evestigatum

Yojiro Ishida, Keiko Inouye, Ouyang Ming, Masayori Inouye

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2 Scopus citations


MazF is a sequence-specific endoribonuclease or mRNA interferase, which cleaves RNA at a specific sequence. Since the expression of a specific gene or a group of specific genes can be regulated by MazF, expanding the repertoire of recognition sequences by MazF mRNA interferases is highly desirable for biotechnological and medical applications. Here, we identified a gene for a MazF homologue (MazFme) from Methanohalobium evestigatum, an extremely halophilic archaeon. In order to suppress the toxicity of MazFme to the E. coli cells, the C-terminal half of the cognate antitoxin MazEme was fused to the N-terminal end of MazFme. Since the fusion of the C-terminal half of MazEme to MazFme was able to neutralize MazFme toxicity, the MazEme-MazFme fusion protein was expressed in a large amount without any toxic effects. After purification of the MazEme, the free MazFme RNA cleavage specificity was determined by primer extension and synthetic ribonucleotides, revealing that MazFme is a CUGGU/UUGGU-specific endoribonuclease.

Original languageEnglish (US)
Pages (from-to)533-540
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - Oct 20 2019

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


  • Archaea
  • Endoribonuclease
  • MazF
  • Toxin-antitoxin (TA)


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