A designer biomimetic vector with a chimeric architecture for targeted gene transfer

Yuhua Wang, Sriramchandra Sastry Mangipudi, Brenda F. Canine, Arash Hatefi

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Designer biomimetic vectors are genetically engineered biomacromolecules that are designed to mimic viral characteristics in order to overcome the cellular barriers associated with the targeted gene transfer. The vector in this study was genetically engineered to contain at precise locations: a) four tandem repeating units of N-terminal domain of histone H2A to condense DNA into stable nanosize particles suitable for cellular uptake, b) a model targeting motif to target HER2 and enhance internalization of nanoparticles, and c) a pH-responsive synthetic fusogenic peptide to disrupt endosome membranes and promote escape of the nanoparticles into the cytosol. The results demonstrate that a fully functional, multi-domain, designer vector can be engineered to target cells with high specificity, overcome the biological barriers associated with targeted gene transfer, and mediate efficient gene transfer.

Original languageEnglish (US)
Pages (from-to)46-53
Number of pages8
JournalJournal of Controlled Release
Volume137
Issue number1
DOIs
StatePublished - Jul 1 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Keywords

  • Biomimetic vector
  • Designer biomacromolecule
  • Gene therapy
  • Non-viral vector

Fingerprint

Dive into the research topics of 'A designer biomimetic vector with a chimeric architecture for targeted gene transfer'. Together they form a unique fingerprint.

Cite this