A DNA damage signal is required for p53 to activate gadd45

Xiao Gu, Agustin Chicas, Magali Olivier, Yoichi Taya, Sanjay Tyagi, Fred Rusell Kramer, Jill Bargonetti

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

We provide direct evidence that overexpression of p53 is not sufficient for robust p53-dependent activation of the endogenous gadd45 gene. When p53 was induced in TR9-7 cells in the absence of DNA damage, waf1/p21 and mdm2 mRNA levels were increased, but a change in gadd45 mRNA was barely detectable. Activation of the gadd45 gene was observed when camptothecin was added to cells containing p53 in the absence of a further increase in the p53 level. Phosphorylation of p53 at serine 15 and acetylation at lysine 382 were detected after drug treatment. It has been suggested that p53 posttranslational modification is critical during activation. However, inhibition of these modifications by wortmannin was not sufficient to block the transactivation of gadd45. Interestingly, after camptothecin treatment, increased DNase I sensitivity was detected at the gadd45 promoter, suggesting that an undetermined DNA damage signal is involved in inducing chromatin remodeling at the gadd45 promoter while cooperating with p53 to activate gadd45 transcription.

Original languageEnglish (US)
Pages (from-to)1711-1719
Number of pages9
JournalCancer Research
Volume60
Issue number6
StatePublished - Mar 15 2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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