A dominant nuclear streptomycin resistance marker for plant cell transformation

Jonathan D.G. Jones, Zora Svab, Elisabeth C. Harper, Charles D. Hurwitz, Pal Maliga

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Plant cells in photoheterotrophic culture respond to streptomycin by bleaching and retarded growth but no cell death. A new genetic marker for plant cell transformation has been developed that is based on the expression of the enzyme streptomycin phosphotransferase (SPT), and confers the ability to form green colonies on a selective medium. Coding sequences of SPT from the bacterial transposon Tn5 were placed under the control of gene expression signals derived from the Agrobacterium Ti plasmid Ach5. The 5′ end of the SPT gene has been replaced with the promoter region of the gene coding for the first enzyme of agropine biosynthesis, the 3′ end with that of the enzyme octopine synthase. The chimeric SPT gene has been linked to a selectable kanamycin resistance gene, and introduced into Nicotiana tabacum and Nicotiana plumbaginifolia by selection for the linked kanamycin resistance marker. Streptomycin resistance was expressed in some but not all of the kanamycin-resistant lines and was transmitted to the seed progeny as a dominant nuclear trait.

Original languageEnglish (US)
Pages (from-to)86-91
Number of pages6
JournalMgg Molecular & General Genetics
Volume210
Issue number1
DOIs
StatePublished - Nov 1987
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

Keywords

  • Chimeric gene
  • Nicotiana
  • Streptomycin phosphotransferase
  • Streptomycin resistance
  • Transformation

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