A fluorometric assay for dihydrofolate reductase

C. A. Lindquist, E. C. Cadman, J. R. Bertino

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A recording fluorometric assay for dihydrofolate reductase is described. The technique measures the appearance of product, tetrahydrofolate, as the reaction proceeds. The assay can be accomplished with as little as 2 pmol of enzyme and is sufficiently sensitive to allow accurate kinetic studies using very low concentrations of substrate. Kinetic studies of both purified and crude preparations of enzyme are reported.

Original languageEnglish (US)
Pages (from-to)20-25
Number of pages6
JournalAnalytical Biochemistry
Volume83
Issue number1
DOIs
StatePublished - Nov 1977
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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