A fluorometric assay for dihydrofolate reductase

C. A. Lindquist; E. C. Cadman; J. R. Bertino

doi:10.1016/0003-2697(77)90504-8

A fluorometric assay for dihydrofolate reductase

C. A. Lindquist, E. C. Cadman, J. R. Bertino

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A recording fluorometric assay for dihydrofolate reductase is described. The technique measures the appearance of product, tetrahydrofolate, as the reaction proceeds. The assay can be accomplished with as little as 2 pmol of enzyme and is sufficiently sensitive to allow accurate kinetic studies using very low concentrations of substrate. Kinetic studies of both purified and crude preparations of enzyme are reported.

Original language	English (US)
Pages (from-to)	20-25
Number of pages	6
Journal	Analytical Biochemistry
Volume	83
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(77)90504-8
State	Published - Nov 1977
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(77)90504-8

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title = "A fluorometric assay for dihydrofolate reductase",

abstract = "A recording fluorometric assay for dihydrofolate reductase is described. The technique measures the appearance of product, tetrahydrofolate, as the reaction proceeds. The assay can be accomplished with as little as 2 pmol of enzyme and is sufficiently sensitive to allow accurate kinetic studies using very low concentrations of substrate. Kinetic studies of both purified and crude preparations of enzyme are reported.",

author = "Lindquist, {C. A.} and Cadman, {E. C.} and Bertino, {J. R.}",

year = "1977",

month = nov,

doi = "10.1016/0003-2697(77)90504-8",

language = "English (US)",

volume = "83",

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journal = "Analytical Biochemistry",

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T1 - A fluorometric assay for dihydrofolate reductase

AU - Lindquist, C. A.

AU - Cadman, E. C.

AU - Bertino, J. R.

PY - 1977/11

Y1 - 1977/11

N2 - A recording fluorometric assay for dihydrofolate reductase is described. The technique measures the appearance of product, tetrahydrofolate, as the reaction proceeds. The assay can be accomplished with as little as 2 pmol of enzyme and is sufficiently sensitive to allow accurate kinetic studies using very low concentrations of substrate. Kinetic studies of both purified and crude preparations of enzyme are reported.

AB - A recording fluorometric assay for dihydrofolate reductase is described. The technique measures the appearance of product, tetrahydrofolate, as the reaction proceeds. The assay can be accomplished with as little as 2 pmol of enzyme and is sufficiently sensitive to allow accurate kinetic studies using very low concentrations of substrate. Kinetic studies of both purified and crude preparations of enzyme are reported.

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U2 - 10.1016/0003-2697(77)90504-8

DO - 10.1016/0003-2697(77)90504-8

M3 - Article

C2 - 920937

AN - SCOPUS:0017753874

SN - 0003-2697

VL - 83

SP - 20

EP - 25

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

A fluorometric assay for dihydrofolate reductase

Abstract

All Science Journal Classification (ASJC) codes

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