A recording fluorometric assay for dihydrofolate reductase is described. The technique measures the appearance of product, tetrahydrofolate, as the reaction proceeds. The assay can be accomplished with as little as 2 pmol of enzyme and is sufficiently sensitive to allow accurate kinetic studies using very low concentrations of substrate. Kinetic studies of both purified and crude preparations of enzyme are reported.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology