A fluorometric method to measure sublobular rates of mixed-function oxidation in the hemoglobin-free perfused rat liver

S. Ji, J. J. Lemasters, R. G. Thurman

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

A new fluorometric method has been developed to measure the relative rates of the 7-ethoxycoumarin O-deethylase activities in periportal and pericentral regions of the liver lobule. The method utilizes a pair of micro-light guides constructed from two strands of 80 μm-diameter glass optical fibers. The micro-light guides are placed in periportal and pericentral regions on the surface of the perfused liver. The increase in tissue fluorescence due to 7-hydroxycoumarin formation from 7-ethoxycoumarin is measured in periportal and pericentral regions by illuminating tissue with light at 360 ± 50 nm and measuring fluorescence at 450 ± 50 nm. Since the steady-state tissue fluorescence due to 7-hydroxycoumarin monitored with a large light guide was found to be directly proportional to the steady-state rate of 7-ethoxycoumarin O-deethylation, the sublobular fluorescence of 7-hydroxycoumarin measured by micro-light guides following infusion of 7-ethoxycoumarin allows one to estimate sublobular rates of the mixed-function oxidation of 7-ethoxycoumarin. The results indicate that the rate of 7-ethoxycoumarin O-deethylation is approximately twice as large in pericentral regions as in periportal areas of the liver.

Original languageEnglish (US)
Pages (from-to)513-516
Number of pages4
JournalMolecular pharmacology
Volume19
Issue number3
StatePublished - 1981
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology

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