@inproceedings{4df4094010d04dbe97d69b531ea6fcb5,
title = "A functional clotting assay to monitor low molecular weight heparin dosage",
abstract = "Low molecular weight heparin (LMWH), an anticoagulant drug, has seen increased use in a variety of clinical situations. Mercuric ion (Hg2+) has been shown to increase procoagulant activity via Tissue Factor (TF) stimulated production of Factor Xa, a pathway that has been linked to many thrombotic disorders. The effects of Hg2+ on heparinized blood may provide valuable insight into the effectiveness of LMWH to counteract TF-induced hypercoagulability. We performed in vitro studies by spiking human citrated whole blood (CWB) with HgCl2 and LMWH at final concentrations of 0.01 % HgCl2, 0.5 U/mL LMWH, and a combination of these agents. The samples (n=19) were incubated at 37° for 10 minutes and recalcified with 0.1 M CaCl2 to initiate clotting. The clotting or recalcification time (RT) was measured with a Sonoclot Coagulation Analyzer. The results indicate that HgCl2 was able to significantly (p<0.0001) reduce the anticoagulant ability of LMWH. The data warrants a reassessment of current LMWH monitoring and therapeutic protocols.",
keywords = "Blood, Coagulation, Drugs, Humans, In vitro, Mercury (metals), Monitoring, Production, Protocols, Time measurement",
author = "Bhatt, {B. A.} and D. Kristol and Spillert, {C. R.}",
note = "Publisher Copyright: {\textcopyright} 2002 IEEE.; 28th IEEE Annual Northeast Bioengineering Conference, NEBC 2002 ; Conference date: 20-04-2002 Through 21-04-2002",
year = "2002",
doi = "10.1109/NEBC.2002.999457",
language = "English (US)",
series = "Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
pages = "45--46",
editor = "Dalia El-Sherif and Karen Moxon and Saravanan Kanakasabai",
booktitle = "Proceedings of the IEEE 28th Annual Northeast Bioengineering Conference, NEBC 2002",
address = "United States",
}