A functional clotting assay to monitor low molecular weight heparin dosage

B. A. Bhatt, D. Kristol, C. R. Spillert

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Low molecular weight heparin (LMWH), an anticoagulant drug, has seen increased use in a variety of clinical situations. Mercuric ion (Hg2+) has been shown to increase procoagulant activity via Tissue Factor (TF) stimulated production of Factor Xa, a pathway that has been linked to many thrombotic disorders. The effects of Hg2+ on heparinized blood may provide valuable insight into the effectiveness of LMWH to counteract TF-induced hypercoagulability. We performed in vitro studies by spiking human citrated whole blood (CWB) with HgCl2 and LMWH at final concentrations of 0.01 % HgCl2, 0.5 U/mL LMWH, and a combination of these agents. The samples (n=19) were incubated at 37° for 10 minutes and recalcified with 0.1 M CaCl2 to initiate clotting. The clotting or recalcification time (RT) was measured with a Sonoclot Coagulation Analyzer. The results indicate that HgCl2 was able to significantly (p<0.0001) reduce the anticoagulant ability of LMWH. The data warrants a reassessment of current LMWH monitoring and therapeutic protocols.

Original languageEnglish (US)
Title of host publicationProceedings of the IEEE 28th Annual Northeast Bioengineering Conference, NEBC 2002
EditorsDalia El-Sherif, Karen Moxon, Saravanan Kanakasabai
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages45-46
Number of pages2
ISBN (Electronic)0780374193
DOIs
StatePublished - 2002
Event28th IEEE Annual Northeast Bioengineering Conference, NEBC 2002 - Philadelphia, United States
Duration: Apr 20 2002Apr 21 2002

Publication series

NameProceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
Volume2002-January
ISSN (Print)1071-121X
ISSN (Electronic)2160-7001

Other

Other28th IEEE Annual Northeast Bioengineering Conference, NEBC 2002
Country/TerritoryUnited States
CityPhiladelphia
Period4/20/024/21/02

All Science Journal Classification (ASJC) codes

  • Bioengineering

Keywords

  • Blood
  • Coagulation
  • Drugs
  • Humans
  • In vitro
  • Mercury (metals)
  • Monitoring
  • Production
  • Protocols
  • Time measurement

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