A GTP-binding protein of Escherichia coli has homology to yeast RAS proteins

J. Ahnn, P. E. March, H. E. Takiff, M. Inouye

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114 Scopus citations

Abstract

The DNA sequence of a gene (era) located immediately downstream of the gene (rnc) encoding ribonuclease III of Escherichia coli was determined and found to encode a protein of 316 aminoa acid residues. The amino acid sequence of this protein, Era, has significant similarity to the yeast RAS proteins. Overexpression of the Era protein was achieved and GTP cross-linking experiments demonstrated that the protein was indeed capable of binding GTP, as are the yeast and mammalian ras gene products. These data indicate that ras-related sequences occur not only in eukaryotes but also in prokaryotes.

Original languageEnglish (US)
Pages (from-to)8849-8853
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number23
DOIs
StatePublished - 1986
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

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