A GTP-binding protein required for secretion rapidly associates with secretory vesicles and the plasma membrane in yeast

Bruno Goud, Antti Salminen, Nancy C. Walworth, Peter J. Novick

Research output: Contribution to journalArticlepeer-review

385 Scopus citations

Abstract

SEC4, one of the 10 genes involved in the final stage of the yeast secretory pathway, encodes a ras-like, GTP-binding protein. In wild-type cells, Sec4 protein is located on the cytoplasmic face of both the plasma membrane and the secretory vesicles in transit to the cell surface. In all post-Golgi blocked sec mutants, Sec4p is predominantly associated with the secretory vesicles that accumulate as a result of the secretory block. Sec4p is synthesized as a soluble protein that rapidly (t 1 2 ≤ 1 min) and tightly associates with secretory vesicles and the plasma membrane by virtue of a conformational change or a covalent modification. These data suggest that Sec4p may function as a "G" protein on the vesicle surface to transduce an intracellular signal needed to regulate transport between the Golgi apparatus and the plasma membrane.

Original languageEnglish (US)
Pages (from-to)753-768
Number of pages16
JournalCell
Volume53
Issue number5
DOIs
StatePublished - Jun 3 1988
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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