A high-resolution molecular-based panel of assays for identification and characterization of human embryonic stem cell lines

Jennifer C. Moore, Sasha Sadowy, Mina Alikani, Alana J. Toro-Ramos, Mavis R. Swerdel, Ronald P. Hart, Rick I. Cohen

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Meticulous characterization of human embryonic stem cells (hESC) is critical to their eventual use in cell-based therapies, particularly in view of the diverse methods for derivation and maintenance of these cell lines. However, characterization methods are generally not standardized and many currently used assays are subjective, making dependable and direct comparison of cell lines difficult. In order to address this problem, we selected 10 molecular-based high-resolution assays as components of a panel for characterization of hESC. The selection of the assays was primarily based on their quantitative or objective (rather than subjective) nature. We demonstrate the efficacy of this panel by characterizing 4 hESC lines, derived in two different laboratories using different derivation techniques, as pathogen free, genetically stable, and able to differentiate into derivatives of all three germ layers. Our panel expands and refines a characterization panel previously proposed by the International Stem Cell Initiative and is another step toward standardized hESC characterization and quality control, a crucial element of successful hESC research and clinical translation.

Original languageEnglish (US)
Pages (from-to)92-106
Number of pages15
JournalStem Cell Research
Volume4
Issue number2
DOIs
StatePublished - Mar 2010

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

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