A highly selective affinity labeling of phosphofructokinase from Cottocomephorus grewingki

I. I. Tulokhonov; A. A. Mustaev; E. F. Zaichikov

A highly selective affinity labeling of phosphofructokinase from Cottocomephorus grewingki

I. I. Tulokhonov, A. A. Mustaev, E. F. Zaichikov

Research output: Contribution to journal › Article › peer-review

Abstract

The 80-kDa subunit of phosphofructokinase from the Baikalian fish Cottocomephorus grewingki was labeled with high selectivity by a two-stage procedure of affinity labeling using p-formylbenzyl fructose-6-phosphate and [γ-³²P] ATP. The extent of labeling depended on the presence of such effectors as AMP or fructose 1,6-diphosphate in the reaction mixture.

Original language	English (US)
Pages (from-to)	370-372
Number of pages	3
Journal	Russian Journal of Bioorganic Chemistry
Volume	24
Issue number	6
State	Published - 1998
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Organic Chemistry

Keywords

Active site
Affinity labeling
Effectors
Phosphofructokinase

Cite this

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title = "A highly selective affinity labeling of phosphofructokinase from Cottocomephorus grewingki",

abstract = "The 80-kDa subunit of phosphofructokinase from the Baikalian fish Cottocomephorus grewingki was labeled with high selectivity by a two-stage procedure of affinity labeling using p-formylbenzyl fructose-6-phosphate and [γ-32P] ATP. The extent of labeling depended on the presence of such effectors as AMP or fructose 1,6-diphosphate in the reaction mixture.",

keywords = "Active site, Affinity labeling, Effectors, Phosphofructokinase",

author = "Tulokhonov, {I. I.} and Mustaev, {A. A.} and Zaichikov, {E. F.}",

year = "1998",

language = "English (US)",

volume = "24",

pages = "370--372",

journal = "Russian Journal of Bioorganic Chemistry",

issn = "1068-1620",

publisher = "Maik Nauka-Interperiodica Publishing",

number = "6",

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T1 - A highly selective affinity labeling of phosphofructokinase from Cottocomephorus grewingki

AU - Tulokhonov, I. I.

AU - Mustaev, A. A.

AU - Zaichikov, E. F.

PY - 1998

Y1 - 1998

N2 - The 80-kDa subunit of phosphofructokinase from the Baikalian fish Cottocomephorus grewingki was labeled with high selectivity by a two-stage procedure of affinity labeling using p-formylbenzyl fructose-6-phosphate and [γ-32P] ATP. The extent of labeling depended on the presence of such effectors as AMP or fructose 1,6-diphosphate in the reaction mixture.

AB - The 80-kDa subunit of phosphofructokinase from the Baikalian fish Cottocomephorus grewingki was labeled with high selectivity by a two-stage procedure of affinity labeling using p-formylbenzyl fructose-6-phosphate and [γ-32P] ATP. The extent of labeling depended on the presence of such effectors as AMP or fructose 1,6-diphosphate in the reaction mixture.

KW - Active site

KW - Affinity labeling

KW - Effectors

KW - Phosphofructokinase

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M3 - Article

AN - SCOPUS:27544465441

SN - 1068-1620

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JO - Russian Journal of Bioorganic Chemistry

JF - Russian Journal of Bioorganic Chemistry

IS - 6

ER -

A highly selective affinity labeling of phosphofructokinase from Cottocomephorus grewingki

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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