A human airway smooth muscle cell line that retains physiological responsiveness

R. A. Panettieri, R. K. Murray, L. R. DePalo, P. A. Yadvish, M. I. Kotlikoff

Research output: Contribution to journalArticlepeer-review

247 Scopus citations

Abstract

We report the development of a nontransformed line of human airway smooth muscle cells retaining smooth muscle-specific contractile protein expression and physiological responsiveness to agonists implicated in inflammatory airway diseases. Specific responses to histamine, leukotrienes, bradykinin, platelet-activating factor, substance P, and thromboxane analogues are demonstrated as well as functional coupling to β-adrenergic receptors. The cell line was characterized using indirect immunofluorescence, as well as electrophoretic separation and immunoblot analysis of smooth muscle-specific actin. Functional responses were assessed by measurements of cytosolic calcium and stimulation of adenosine 3',5'-cyclic monophosphate production. The cells retain their responsiveness over many population doublings and should be a useful model to examine specific receptor-effector mechanisms, as well as the effects of neurohumoral agents on the regulation of airway smooth muscle growth and differentiation.

Original languageEnglish (US)
Pages (from-to)25/2
JournalAmerican Journal of Physiology - Cell Physiology
Volume256
Issue number2
StatePublished - 1989

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cell Biology

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