A long terminal repeat retrotransposon Cgret from the phytopathogenic fungus Colletotrichum gloeosporioides on cranberry

Peiliang Zhu, Peter V. Oudemans

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

A repetitive DNA element cloned from the cranberry fruit rot pathogen Colletotrichum gloeosporioides has been characterized. Sequence data indicate that it is a long terminal repeat (LTR) retrotransposon of 7,916 base pairs. LTR of 544 base pairs occur at either end of an internal region of 6,828 base pairs. This element, designated Cgret (C. gloeosporioides retrotransposon), encodes two putative polypeptides which have high homology to the gag and pol genes of other fungal retrotransposons. The sequence and structure suggest that Cgret is a member of the gypsy group of LTR retrotransposons. The Cgret retrotransposon was present in all of the cranberry isolates of the fungus C. gloeosporioides from New Jersey and Massachusetts, but not in the cranberry isolates from Wisconsin or Chile. Polymorphisms were detected among field isolates of C. gloeosporioides from various hosts, using hybridization probes derived from the LTR and the reverse transcriptase domain of Cgret. The structural integrity of Cgret suggests that it is still a functional retrotransposon and may be used as a molecular marker to study the genetic diversity distribution of this fungal pathogen.

Original languageEnglish (US)
Pages (from-to)241-247
Number of pages7
JournalCurrent Genetics
Volume38
Issue number5
DOIs
StatePublished - 2000

All Science Journal Classification (ASJC) codes

  • Genetics

Keywords

  • Colletotrichum gloeosporioides
  • Cranberry
  • DNA fingerprint
  • LTR retrotransposon

Fingerprint Dive into the research topics of 'A long terminal repeat retrotransposon Cgret from the phytopathogenic fungus Colletotrichum gloeosporioides on cranberry'. Together they form a unique fingerprint.

Cite this