TY - JOUR
T1 - A member of the Hsp70 family is localized in mitochondria and resembles Escherichia coli DnaK
AU - Leustek, T.
AU - Dalie, B.
AU - Amir-Shapira, D.
AU - Brot, N.
AU - Weissbach, H.
PY - 1989
Y1 - 1989
N2 - A 71-kDa protein (P71) with properties similar to those of the Escherichia coli heat shock protein DnaK has been found in extracts of HeLa cells. P71 was copurified by ATP-agarose affinity chromatography with three additional proteins of the Hsp70 family. Of these proteins, only P71 crossreacted strongly with antiserum raised against purified DnaK, and both DnaK and P71 could be phosphorylated in vitro with [γ-32]ATP in a reaction that was markedly stimulated by Ca2+. In HeLa cells, P71 was found to be concentrated in mitochondria. A protein similar to P71 was also found in calf liver and yeast mitochondria.
AB - A 71-kDa protein (P71) with properties similar to those of the Escherichia coli heat shock protein DnaK has been found in extracts of HeLa cells. P71 was copurified by ATP-agarose affinity chromatography with three additional proteins of the Hsp70 family. Of these proteins, only P71 crossreacted strongly with antiserum raised against purified DnaK, and both DnaK and P71 could be phosphorylated in vitro with [γ-32]ATP in a reaction that was markedly stimulated by Ca2+. In HeLa cells, P71 was found to be concentrated in mitochondria. A protein similar to P71 was also found in calf liver and yeast mitochondria.
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U2 - 10.1073/pnas.86.20.7805
DO - 10.1073/pnas.86.20.7805
M3 - Article
C2 - 2682628
AN - SCOPUS:0024363889
SN - 0027-8424
VL - 86
SP - 7805
EP - 7808
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 20
ER -