A method for recovery of high-molecular-weight DNA suitable for field-inversion gel electrophoresis from frozen tumor cells

Jennifer E. Reynolds, Scott R. Diehl

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

We describe a simple method for isolation of high-molecular-weight (high-mol-wt, >800 kilobases) DNA from tumor cell lines frozen without dimethylsulfoxide (DMSO) or other protective agents. This method should be applicable to most frozen tissues and useful for molecular genetic studies such as field-inversion gel electrophoresis (FIGE), or cloning procedures that require access to intact, high-mol-wt DNA.

Original languageEnglish (US)
Pages (from-to)68-70
Number of pages3
JournalCancer Genetics and Cytogenetics
Volume65
Issue number1
DOIs
StatePublished - Jan 1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

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