A method for recovery of high-molecular-weight DNA suitable for field-inversion gel electrophoresis from frozen tumor cells

Jennifer E. Reynolds, Scott Diehl

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We describe a simple method for isolation of high-molecular-weight (high-mol-wt, >800 kilobases) DNA from tumor cell lines frozen without dimethylsulfoxide (DMSO) or other protective agents. This method should be applicable to most frozen tissues and useful for molecular genetic studies such as field-inversion gel electrophoresis (FIGE), or cloning procedures that require access to intact, high-mol-wt DNA.

Original languageEnglish (US)
Pages (from-to)68-70
Number of pages3
JournalCancer Genetics and Cytogenetics
Volume65
Issue number1
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

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Pulsed Field Gel Electrophoresis
Molecular Weight
DNA
Protective Agents
Neoplasms
Dimethyl Sulfoxide
Tumor Cell Line
Organism Cloning
Molecular Biology

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

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A method for recovery of high-molecular-weight DNA suitable for field-inversion gel electrophoresis from frozen tumor cells. / Reynolds, Jennifer E.; Diehl, Scott.

In: Cancer Genetics and Cytogenetics, Vol. 65, No. 1, 01.01.1993, p. 68-70.

Research output: Contribution to journalArticle

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