A microELISA Raji cell assay to detect immune complexes

P. K. Coyle, Nancy Banks, Steven E. Schutzer

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The Raji cell assay to detect immune complexes has been modified to a microtiter ELISA system. Raji cells were fixed to microplate wells, then reacted with serum samples or aggregated human IgG. Horseradish peroxidase-conjugated anti-human IgG was used to detect bound complexes. There was a linear relationship between aggregated IgG added and optical density reading, with less than 2 μg/ml of aggregated IgG readily detected. When applied to human serum this technique gave results comparable to those obtained with the standard Raji cell assay. The Raji micro-ELISA is simpler to perform than the standard assay, is equally reliable, and avoids the hazards of radioactivity.

Original languageEnglish (US)
Pages (from-to)191-197
Number of pages7
JournalJournal of Immunological Methods
Volume74
Issue number1
DOIs
StatePublished - Nov 16 1984
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Keywords

  • Raji cell
  • immune complexes
  • microELISA assay

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