A microsomal enzyme inhibitor in the gut contents of the house cricket (Acheta domesticus)

L. B. Brattsten, C. F. Wilkinson

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

1. 1. An inhibitor of microsomal oxidation was isolated and partially purified from the soluble fraction of house cricket (Acheta domesticus) gut contents. 2. 2. Inhibitory activity was greater towards armyworm gut microsomal enzymes than those from rat liver. 3. 3. The material was characterized as a proteolytic enzyme with a molecular weight of approximately 16,500. 4. 4. Proteolytic and inhibitory activity were blocked by Soy trypsin inhibitor and phenylmethanesulfonyl fluoride but not by p-chloromercuribenzoate or reduced glutathione. 5. 5. Inhibition apparently results from solubilization of NADPH cytochrome c reductase from the microsomal membrane.

Original languageEnglish (US)
Pages (from-to)59-70
Number of pages12
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume45
Issue number1
DOIs
StatePublished - May 15 1973
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology

Keywords

  • Acheta domesticus
  • Microsomal enzyme inhibitor
  • NADPH-cytochrome c reductase

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