A mimetic peptide of α2,6-sialyllactose promotes neuritogenesis

Shuang Xi Chen, Jia Hui He, Yong Jian Mi, Hui Fan Shen, Melitta Schachner, Wei Jiang Zhao

Research output: Contribution to journalArticle

Abstract

Oxidative stress contributes to the pathogenesis of neurodegenerative diseases. With the aim to find reagents that reduce oxidative stress, a phage display library was screened for peptides mimicking α2,6-sialyllactose (6′-SL), which is known to beneficially influence neural functions. Using Sambucus nigra lectin, which specifically binds to 6′-SL, we screened a phage display library and found a peptide comprising identical sequences of 12 amino acids. Mimetic peptide, reverse peptide and scrambled peptide were tested for inhibition of 6′-SL binding to the lectin. Indeed, lectin binding to 6′-SL was inhibited by the most frequently identified mimetic peptide, but not by the reverse or scrambled peptides, showing that this peptide mimics 6′-SL. Functionally, mimetic peptide, but not the reverse or scrambled peptides, increased viability and expression of neural cell adhesion molecule L1 in SK-N-SH human neuroblastoma cells, and promoted survival and neurite outgrowth of cultured mouse cerebellar granule neurons challenged by H2O2-induced oxidative stress. The combined results indicate that the 6′-SL mimetic peptide promotes neuronal survival and neuritogenesis, thus raising hopes for the treatment of neurodegenerative diseases. This study was approved by the Medical Ethics Committee of Shantou University Medical College, China (approval No. SUMC 2014-004) on February 20, 2014.

Original languageEnglish (US)
Pages (from-to)1058-1065
Number of pages8
JournalNeural Regeneration Research
Volume15
Issue number6
DOIs
StatePublished - Jun 1 2020

Fingerprint

Peptides
Oxidative Stress
Lectins
Neurodegenerative Diseases
Bacteriophages
Libraries
Hope
2,6-sialyllactose
Neural Cell Adhesion Molecule L1
Ethics Committees
Medical Ethics
Neuroblastoma
6'-sialyllactose
Amino Acid Sequence
China
Cell Survival
Neurons

All Science Journal Classification (ASJC) codes

  • Developmental Neuroscience

Keywords

  • 6-sialyllactose
  • central nervous system
  • cerebellar granule neurons
  • mimetic peptide
  • neural cell adhesion molecule L1
  • neuritogenesis
  • neurodegenerative disease
  • neuronal survival
  • oxidative stress
  • phage display
  • Sambucus nigra lectin
  • α2

Cite this

Chen, Shuang Xi ; He, Jia Hui ; Mi, Yong Jian ; Shen, Hui Fan ; Schachner, Melitta ; Zhao, Wei Jiang. / A mimetic peptide of α2,6-sialyllactose promotes neuritogenesis. In: Neural Regeneration Research. 2020 ; Vol. 15, No. 6. pp. 1058-1065.
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A mimetic peptide of α2,6-sialyllactose promotes neuritogenesis. / Chen, Shuang Xi; He, Jia Hui; Mi, Yong Jian; Shen, Hui Fan; Schachner, Melitta; Zhao, Wei Jiang.

In: Neural Regeneration Research, Vol. 15, No. 6, 01.06.2020, p. 1058-1065.

Research output: Contribution to journalArticle

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AU - He, Jia Hui

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