TY - JOUR
T1 - A mixture of citrus polymethoxyflavones, green tea polyphenols and lychee extracts attenuates adipogenesis in 3T3-L1 adipocytes and obesity-induced adipose inflammation in mice
AU - Pan, Min Hsiung
AU - Li, Ming Yi
AU - Tsai, Mei Ling
AU - Pan, Chih Yu
AU - Badmaev, Vladimir
AU - Ho, Chi Tang
AU - Lai, Ching Shu
N1 - Funding Information:
This study was supported by the MOST 105-2320-B-002-031- MY3 and 105-2320-B-022-001.
Funding Information:
aInstitute of Food Science and Technology, National Taiwan University, Taipei 10617, Taiwan bDepartment of Medical Research, China Medical University Hospital, China Medical University, Taichung 40402, Taiwan cDepartment of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan dDepartment of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung 811, Taiwan. E-mail: [email protected] eKaohsiung St. Paul’s High School, Kaohsiung 804, Taiwan fAmerican Medical Holdings Incorporated, New York, USA gDepartment of Food Science, Rutgers University, New Brunswick, NJ 08901, USA
Funding Information:
This study was supported by the MOST 105-2320-B-002-031-MY3 and 105-2320-B-022-001.
Publisher Copyright:
© 2019 The Royal Society of Chemistry.
PY - 2019/12
Y1 - 2019/12
N2 - Adipocyte-macrophage interaction in obesity can cause adipose tissue inflammation and contribute to insulin resistance. Here, we investigated the effect of SlimTrym® - a formulated product containing citrus polymethoxyflavones (PMFs), green tea extract, and lychee polyphenols - on 3T3-L1 adipocyte differentiation and obesity-induced inflammation. SlimTrym® inhibited mitotic clonal expansion (MCE) of 3T3-L1 adipocytes by inducing G1 cell cycle arrest via upregulation of p21 and p53. SlimTrym® attenuated adipogenic differentiation by downregulating adipogenic factors, such as CCAAT-enhancer-binding proteins (C/EBPs) and peroxisome proliferator-activated receptor γ (PPARγ), and upregulating AMP-activated protein kinase (AMPK). Pretreatment with compound C significantly reduced SlimTrym®-mediated suppression of lipid accumulation. SlimTrym® reduced the expression of pro-inflammatory cytokines, including monocyte chemoattractant protein 1 (MCP-1), interleukin (IL)-1β and IL-6, in co-cultured 3T3-L1 adipocytes and RAW264.7 macrophages. C57BL/6 mice administered with SlimTrym® for 16 weeks showed markedly reduced high-fat diet (HFD)-induced infiltration of monocytes/macrophages in adipose tissue; however, the level of M2 macrophage markers (CD163 and IL-10) was increased. Taken together, these findings indicate that SlimTrym® exerts both anti-adipogenic and anti-inflammatory effects, and can potentially treat obesity and adipose tissue inflammation.
AB - Adipocyte-macrophage interaction in obesity can cause adipose tissue inflammation and contribute to insulin resistance. Here, we investigated the effect of SlimTrym® - a formulated product containing citrus polymethoxyflavones (PMFs), green tea extract, and lychee polyphenols - on 3T3-L1 adipocyte differentiation and obesity-induced inflammation. SlimTrym® inhibited mitotic clonal expansion (MCE) of 3T3-L1 adipocytes by inducing G1 cell cycle arrest via upregulation of p21 and p53. SlimTrym® attenuated adipogenic differentiation by downregulating adipogenic factors, such as CCAAT-enhancer-binding proteins (C/EBPs) and peroxisome proliferator-activated receptor γ (PPARγ), and upregulating AMP-activated protein kinase (AMPK). Pretreatment with compound C significantly reduced SlimTrym®-mediated suppression of lipid accumulation. SlimTrym® reduced the expression of pro-inflammatory cytokines, including monocyte chemoattractant protein 1 (MCP-1), interleukin (IL)-1β and IL-6, in co-cultured 3T3-L1 adipocytes and RAW264.7 macrophages. C57BL/6 mice administered with SlimTrym® for 16 weeks showed markedly reduced high-fat diet (HFD)-induced infiltration of monocytes/macrophages in adipose tissue; however, the level of M2 macrophage markers (CD163 and IL-10) was increased. Taken together, these findings indicate that SlimTrym® exerts both anti-adipogenic and anti-inflammatory effects, and can potentially treat obesity and adipose tissue inflammation.
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U2 - 10.1039/c9fo02235j
DO - 10.1039/c9fo02235j
M3 - Article
C2 - 31793969
AN - SCOPUS:85076382414
SN - 2042-6496
VL - 10
SP - 7667
EP - 7677
JO - Food and Function
JF - Food and Function
IS - 12
ER -