A modified protocol for the high yield production of a recombinant adeno-associated virus vector

Zhengyun Jiang, Yicong Le, Min Chen, Yang Yang, Xuetao Xu, Chuan Li, Kun Zhang, Suqing Zhao, Renping Zhou

Research output: Contribution to journalArticlepeer-review

Abstract

Recombinant adeno-associated viruses (rAAVs) have been widely used in gene therapy clinical trials and preclinical investigations. There is a significant need for the improvement of current laboratory protocols for the production of rAAV. In the modified protocol, only the cell culture medium was used for rAAV purification, while the cell culture and rAAV purification methods were modified. The rAAV production yield increased six-fold compared to the standard method, while the recovery rate increased by 42.06% and the rAAV infection efficiency improved by 18.88%. This modified rAAV vector production protocol has the advantages of higher yield, higher recovery rate, and higher infection efficiency. It has been tailored toward the needs of rAAV gene therapy laboratories involved in preclinical trials and can be used for diverse in vitro and in vivo preclinical applications.

Original languageEnglish (US)
Article number101227
JournalGene Reports
Volume24
DOIs
StatePublished - Sep 2021

All Science Journal Classification (ASJC) codes

  • Genetics

Keywords

  • Adeno-associated virus production
  • Gene therapy
  • Recombinant adeno-associated virus
  • Viral vector

Fingerprint

Dive into the research topics of 'A modified protocol for the high yield production of a recombinant adeno-associated virus vector'. Together they form a unique fingerprint.

Cite this