A negative selection scheme based on the expression of cytosine deaminase in plastids

German Serino, Pal Maliga

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

The enzyme cytosine deaminase (CD) encoded by coda catalyzes deamination of cytosine to uracil. CD is present in prokaryotes and in many eukaryotic micro-organisms, but is absent in higher plants. 5-fluorocytosine (5FC) is metabolized in CD-expressing cells, causing cellular death. A chimeric coda has been introduced into the tobacco plastid genome and 5FC was used to select against tissue culture cells and seedlings expressing CD. This negative selection scheme will be useful in identifying nuclear genes which control plastid gene expression in higher plants.

Original languageEnglish (US)
Pages (from-to)697-701
Number of pages5
JournalPlant Journal
Volume12
Issue number3
DOIs
StatePublished - 1997

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

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