A new approach for the reaction of Sepharose with cyanogen bromide is described, using triethylamine as a "cyano-transfer" reagent. An optimized procedure for activation at neutral pH was developed. This procedure requires only about 5% of the usual amount of cyanogen bromide. Activated resins are free of imidocarbonates and carbamates, containing only active cyanate esters. Extremely high coupling capacities (75 μmol ligand/g wet Sepharose 4B) can be obtained using this method.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Aug 16 1982|
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology