A new approach for quantitation of receptors and their ligands

Debkumar Pain, Avadhesha Surolia

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

A novel and sensitive method for quantitating receptors and their ligands is described, using the calf uterine estrogen receptor and estradiol as a model system. When ligand conjugated to malate dehydrogenase is incubated with the estrogen receptor, the enzyme is inhibited proportionately to the concentration of receptor. However, receptor saturated with free ligand has almost no effect on the ligand-conjugated enzyme. The assay can detect as little as three femtomoles of receptor and one femtomole of estradiol and can, in principle, be applied to any receptor-ligand interaction.

Original languageEnglish (US)
Pages (from-to)419-425
Number of pages7
JournalJournal of molecular biology
Volume143
Issue number4
DOIs
StatePublished - Jan 1 1980
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

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