A new approach to assessing sweat production

Research output: Contribution to journalArticle


Since 1916 when Stillians (1) first reported that excessive axillary sweating could be reduced by the application of 25% aqueous aluminium chloride, sales of antiperspirant products have increased dramatically. In spite of many studies, the mechanism of action of these compounds has not been clearly elucidated (2). In most cases, investigations have been performed on animal foot pads, excised human biopsy specimens and skin samples in vivo (3). A model which eliminated problems encountered in whole body investigations (for example, influence of blood flow) would be preferable. One such method, introduced in part by Sato (3), has been investigated. Preliminary investigations suggest that this method could become useful in the evaluation of the mode of action of antiperspirants. Sweat production was induced using carbachol and methacholine in isolated rat plantar sweat glands. These were maintained in a suitable environment (modified Krebs‐Ringer bicarbonate at 38°C containing 30% w/v fresh rat serum). The output of sweat was measured every 5 minutes for 85 minutes and the maximal final volume obtained with any one gland was 23 nanolitres. Sweat production ceased on administration of appropriate concentrations of atropine or the withdrawal of calcium ions from the bathing fluid. Une nouvelle approche pour évaluer la transpiration

Original languageEnglish (US)
Pages (from-to)267-275
Number of pages9
JournalInternational Journal of Cosmetic Science
Issue number6
Publication statusPublished - Jan 1 1983
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Chemistry (miscellaneous)
  • Aging
  • Pharmaceutical Science
  • Drug Discovery
  • Dermatology
  • Colloid and Surface Chemistry


  • Isolated eccrine sweat gland
  • atropine
  • calcium
  • carbachol
  • methacholine
  • rat
  • sweat production

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