A non-essential domain of Escherichia coli RNA polymerase required for the action of the termination factor ale

K. Severinov, M. Kashlev, E. Severinova, I. Bass, K. McWilliams, E. Kutter, V. Nikiforov, L. Snyder, A. Goldfarb

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

An evolutionarily nonconserved region of ~250 amino-acids can be deleted from the amino-terminal part of the β subunit of Escherichia coli RNA polymerase without effect on the enzyme's basic function. The non-essential segment is located between two highly conserved motifs and is flanked by sequences participating in the rifampicin-binding site. The results define the second non-essential domain in the β subunit, in addition to the more distal dispensable segment identified previously. The Alc protein of bacteriophage T4 participates in the host transcription shutoff after infection by causing premature termination of transcription on E. coli DNA. Point mutations which prevent Alc action in vivo change amino acids in the non-essential NH2-terminal domain of the β subunit. These point mutations as well as deletions which remove the non-essential region also prevent Alc action. Thus, in the RNA polymerase molecule, the proximal non-essential domain of β may function as an acceptor of Alc or other regulatory factors.

Original languageEnglish (US)
Pages (from-to)14254-14259
Number of pages6
JournalJournal of Biological Chemistry
Volume269
Issue number19
StatePublished - Jan 1 1994

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DNA-Directed RNA Polymerases
Transcription
Point Mutation
Escherichia coli
Amino Acids
Rifampin
Binding Sites
Molecules
DNA
Enzymes
Infection
Enterobacteria phage T4 Alc protein

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Severinov, K., Kashlev, M., Severinova, E., Bass, I., McWilliams, K., Kutter, E., ... Goldfarb, A. (1994). A non-essential domain of Escherichia coli RNA polymerase required for the action of the termination factor ale. Journal of Biological Chemistry, 269(19), 14254-14259.
Severinov, K. ; Kashlev, M. ; Severinova, E. ; Bass, I. ; McWilliams, K. ; Kutter, E. ; Nikiforov, V. ; Snyder, L. ; Goldfarb, A. / A non-essential domain of Escherichia coli RNA polymerase required for the action of the termination factor ale. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 19. pp. 14254-14259.
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Severinov, K, Kashlev, M, Severinova, E, Bass, I, McWilliams, K, Kutter, E, Nikiforov, V, Snyder, L & Goldfarb, A 1994, 'A non-essential domain of Escherichia coli RNA polymerase required for the action of the termination factor ale', Journal of Biological Chemistry, vol. 269, no. 19, pp. 14254-14259.

A non-essential domain of Escherichia coli RNA polymerase required for the action of the termination factor ale. / Severinov, K.; Kashlev, M.; Severinova, E.; Bass, I.; McWilliams, K.; Kutter, E.; Nikiforov, V.; Snyder, L.; Goldfarb, A.

In: Journal of Biological Chemistry, Vol. 269, No. 19, 01.01.1994, p. 14254-14259.

Research output: Contribution to journalArticle

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