A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay

Walter J. Miller, Mark L. Kayton, Angela Patton, Sarah O'Connor, Mei He, Huan Vu, Galina Baibakov, Dominique Lorang, Vladimir Knezevic, Elise Kohn, H. Richard Alexander, David Stirling, Faribourz Payvandi, George W. Muller, Steven K. Libutti

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Reliable quantitative evaluation of molecular pathways is critical for both drug discovery and treatment monitoring. We have modified the CAM assay to quantitatively measure vascular density, endothelial proliferation, and changes in protein expression in response to anti-angiogenic and pro-angiogenic agents. This improved CAM assay can correlate changes in vascular density with changes seen on a molecular level. We expect that these described modifications will result in a single in vivo assay system, which will improve the ability to investigate molecular mechanisms underlying the angiogenic response.

Original languageEnglish (US)
Article number4
JournalJournal of translational medicine
Volume2
DOIs
StatePublished - Jan 30 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Keywords

  • Angiogenesis
  • Chorioallantoic membrane (CAM)
  • Confocal microscopy
  • Layered Expression Scanning (LES)
  • XTT assay

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    Miller, W. J., Kayton, M. L., Patton, A., O'Connor, S., He, M., Vu, H., Baibakov, G., Lorang, D., Knezevic, V., Kohn, E., Alexander, H. R., Stirling, D., Payvandi, F., Muller, G. W., & Libutti, S. K. (2004). A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay. Journal of translational medicine, 2, [4]. https://doi.org/10.1186/1479-5876-2-4