A peptide isolated from phage display libraries is a structural and functional mimic of an RGD-binding site on integrins

Renata Pasqualini, Erkki Koivunen, Erkki Ruoslahti

Research output: Contribution to journalArticlepeer-review

189 Scopus citations


Many integrins recognize short RGD-containing amino acid sequences and such peptide sequences can be identified from phage libraries by panning with an integrin. Here, in a reverse strategy, we have used such libraries to isolate minimal receptor sequences that bind to fibronectin and RGD- containing fibronectin fragments in affinity panning. A predominant cyclic motif, *CWDD(G)/(L)WLC*, was obtained (the asterisks denote a potential disulfide bond). Studies using the purified phage and the corresponding synthetic cyclic peptides showed that *CWDDGWLC*-expressing phage binds specifically to fibronectin and to fibronectin fragments containing the RGD sequence. The binding did not require divalent cations and was inhibited by both RGD and *CWDDGWLC*-containing synthetic peptides. Conversely, RGD- expressing phage attached specifically to immobilized *CWDDGWLC*-peptide and the binding could be blocked by the respective synthetic peptides in solution. Moreover, fibronectin bound to a *CWDDGWLC*-peptide affinity column, and could be eluted with an RGD-containing peptide. The *CWDDGWLC*- peptide inhibited RGD-dependent cell attachment to fibronectin and vitronectin, but not to collagen. A region of the β subunit of RGD-binding integrins that has been previously demonstrated to be involved in ligand binding includes a polypeptide stretch, KDDLW (in β3) similar to WDD(G)/(L)WL. Synthetic peptides corresponding to this region in β3 were found to bind RGD-displaying phage and conversion of its two aspartic residues into alanines greatly reduced the RGD binding. Polyclonal antibodies raised against the *CWDDGWLC*-peptide recognized β1 and β3 in immunoblots. These data indicate that the *CWDDGWLC*-peptide is a functional mimic of ligand binding sites of RGD-directed integrins, and that the structurally similar site in the integrin β subunit is a binding site for RGD.

Original languageEnglish (US)
Pages (from-to)1189-1196
Number of pages8
JournalJournal of Cell Biology
Issue number5
StatePublished - Sep 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cell Biology

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