A preliminary study on the receptor for axon guidance factor Netrin-4

Ke Ke Yu, Shu Tong Qin, Si Ying Wang, Ren Ping Zhou, Cheng Gang Zhang

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To study the receptors for the axon guidance factor Netrin-4 and whether the known Netrin-1 receptors, DCC and UNC5H1, are coreceptors for Netrin-4. Methods: The receptor for Netrin-4 was detected by affinity-cytochemistry and in situ staining with the alkaline phosphatase (AP) tagged Netrin-4 fusion protein. The dynamic character of the binding between Netrin-4 and its receptor was evaluated through ligand-receptor binding assay and competitive binding assay. Results: In situ staining of AP4-Netrin-4 protein bound to COS7 cells transiently transfected with eukaryotic expression coding DCC and UNC5H1 showed that the Netrin4 could bind to the transfected cells. Competitive binding assays showed that Netrin-1 could compete with Netrin-4 for the same receptors. Conclusion: DCC and UNC5H1 are coreceptors for Netrin-4. However, because the binding affinity of Netrin-4 with its receptor was lower than that of Netrin-1, it could be inferred that there should exist unique receptor(s) for Netrin-4 other than DCC and UNC5H1.

Original languageEnglish (US)
Pages (from-to)175-181
Number of pages7
JournalNeuroscience Bulletin
Volume21
Issue number3
StatePublished - Dec 1 2005

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Physiology

Keywords

  • Axon guidance
  • DCC
  • Netrin-4
  • Receptor
  • UNC5H

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