A radiation hybrid map spanning the entire human X Chromosome integrating YACs, genes, and STS markers

J. Kumlien, A. Grigoriev, H. Roest Crollius, M. Ross, P. N. Goodfellow, H. Lehrach

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

We present a radiation hybrid (RH) map of human Chromosome (Chr) X, using 50 markers on 72 radiation hybrids. The markers, obtained from the consensus map, form a grid spanning the entire chromosome. To check the RH map, the marker order was determined by analysis of presence or absence of retained human DNA fragments in the RHs: the comparison with the consensus showed a similar order. Any STSs, microsatellites, genes, and clones can be positioned and ordered relative to the marker grid. This approach integrates genetic, physical, and large-scale clone mapping and is used to link YAC contigs containing data from various experimental sources.

Original languageEnglish (US)
Pages (from-to)758-766
Number of pages9
JournalMammalian Genome
Volume7
Issue number10
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

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