A semiquantitative PCR technique for detecting chimerism in hamster-to-rat bone marrow xenotransplantation

Ruhul H. Kuddus, Y. H. Lee, Luis A. Valdivia

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Although bone marrow transplantation has been used to induce donor-specific tolerance in many allogeneic models, similar effort in xenogeneic transplantation is met with obstacles like more severe graft versus host disease (GVHD). We are currently engaged in developing a GVHD-free hamster-to-rat xenotransplantation model using splenectomy, total body irradiation, and donor bone marrow transplantation. To test donor cell chimerism, particularly in the solid tissues, we developed a semiquantitative polymerase chain reaction (PCR) method using primers specific for hamster β-actin and mitochondrial cytochrome C oxidase I and II (MCO I and II) genes and rat sex determination region on the Y chromosome (SRY) gene. Using this method, we estimated the level of hamster cells chimerism in rats subjected to splenectomy, total body irradiation (10 Gy), and hamster bone marrow transplantation (3×108 cell/recipient) and observed high levels of donor cells in all recipient tissues tested.

Original languageEnglish (US)
Pages (from-to)245-251
Number of pages7
JournalJournal of Immunological Methods
Volume285
Issue number2
DOIs
StatePublished - Feb 15 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Keywords

  • Bone marrow
  • Chimera
  • Graft versus host disease
  • Hamster
  • PCR
  • Rat
  • Xenotransplantation

Fingerprint

Dive into the research topics of 'A semiquantitative PCR technique for detecting chimerism in hamster-to-rat bone marrow xenotransplantation'. Together they form a unique fingerprint.

Cite this