A simple, general procedure for purifying restriction endonucleases

Thomas A. Bickle; Vincenzo Pirrotta; Roland Imber

doi:10.1093/nar/4.8.2561

A simple, general procedure for purifying restriction endonucleases

Thomas A. Bickle, Vincenzo Pirrotta, Roland Imber

Research output: Contribution to journal › Article › peer-review

140 Scopus citations

Abstract

A simple, general method for purifying restriction endonucleases is described. The method employs precipitation of nucleic acids from crude extracts with polyethyleneimine followed by affinity chromatography on columns of heparin covalently linked to agarose. Most of the sixteen enzymes tested could be purified to a degree sufficient for DNA sequencing work by this method sometimes supplemented by at most one step of ion exchange chromatography.

Original language	English (US)
Pages (from-to)	2561-2572
Number of pages	12
Journal	Nucleic acids research
Volume	4
Issue number	8
DOIs	https://doi.org/10.1093/nar/4.8.2561
State	Published - Aug 1977
Externally published	Yes

All Science Journal Classification (ASJC) codes

Genetics

Access to Document

10.1093/nar/4.8.2561

Cite this

@article{f45ad2d371df4e36a165deae05f00def,

title = "A simple, general procedure for purifying restriction endonucleases",

abstract = "A simple, general method for purifying restriction endonucleases is described. The method employs precipitation of nucleic acids from crude extracts with polyethyleneimine followed by affinity chromatography on columns of heparin covalently linked to agarose. Most of the sixteen enzymes tested could be purified to a degree sufficient for DNA sequencing work by this method sometimes supplemented by at most one step of ion exchange chromatography.",

author = "Bickle, {Thomas A.} and Vincenzo Pirrotta and Roland Imber",

note = "Funding Information: ACKNOWLEDGMENTS We would like to thank R.3. Roberts for bacterial strains and unpublished information and S. Clarkson for T^. aquaticus cells and information concerning the Sst purification. This work was partly supported by grants from the Swiss National Foundation for Scientific Research.",

year = "1977",

month = aug,

doi = "10.1093/nar/4.8.2561",

language = "English (US)",

volume = "4",

pages = "2561--2572",

journal = "Nucleic acids research",

issn = "0305-1048",

publisher = "Oxford University Press",

number = "8",

}

TY - JOUR

T1 - A simple, general procedure for purifying restriction endonucleases

AU - Bickle, Thomas A.

AU - Pirrotta, Vincenzo

AU - Imber, Roland

N1 - Funding Information: ACKNOWLEDGMENTS We would like to thank R.3. Roberts for bacterial strains and unpublished information and S. Clarkson for T^. aquaticus cells and information concerning the Sst purification. This work was partly supported by grants from the Swiss National Foundation for Scientific Research.

PY - 1977/8

Y1 - 1977/8

N2 - A simple, general method for purifying restriction endonucleases is described. The method employs precipitation of nucleic acids from crude extracts with polyethyleneimine followed by affinity chromatography on columns of heparin covalently linked to agarose. Most of the sixteen enzymes tested could be purified to a degree sufficient for DNA sequencing work by this method sometimes supplemented by at most one step of ion exchange chromatography.

AB - A simple, general method for purifying restriction endonucleases is described. The method employs precipitation of nucleic acids from crude extracts with polyethyleneimine followed by affinity chromatography on columns of heparin covalently linked to agarose. Most of the sixteen enzymes tested could be purified to a degree sufficient for DNA sequencing work by this method sometimes supplemented by at most one step of ion exchange chromatography.

UR - http://www.scopus.com/inward/record.url?scp=0017643983&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017643983&partnerID=8YFLogxK

U2 - 10.1093/nar/4.8.2561

DO - 10.1093/nar/4.8.2561

M3 - Article

C2 - 909783

AN - SCOPUS:0017643983

SN - 0305-1048

VL - 4

SP - 2561

EP - 2572

JO - Nucleic acids research

JF - Nucleic acids research

IS - 8

ER -

A simple, general procedure for purifying restriction endonucleases

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this