A simple method for isolating disomic strains of Saccharomyces cerevisiae

David C. Zebrowski, David B. Kaback

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

A simple method to select disomic (N + 1) strains that should be applicable for almost any chromosome in Saccharomyces cerevisiae is presented. A diploid heterozygous for a KanMX knock-out mutation in an essential gene is sporulated and viable geneticin (G418)-resistant colonies selected. Disomic products of a missegregation or non-disjunction event containing a copy of both the wild-type essential gene and its complementary KanMX knock-out allele make up most of the viable colonies. This method has been used to isolate disomic haploids for a variety of chromosomes. It is appropriately named MARV (for missegregation-associated restoration of viability) and is easily adaptable to virtually any strain.

Original languageEnglish (US)
Pages (from-to)321-326
Number of pages6
JournalYeast
Volume25
Issue number5
DOIs
StatePublished - May 2008

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Genetics

Keywords

  • Aneuploid
  • Yeast

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