A simplified procedure for the quantitative isolation of DNA and RNA transcripts synthesized in vitro

M. J. Modak

doi:10.1016/0003-2697(76)90087-7

A simplified procedure for the quantitative isolation of DNA and RNA transcripts synthesized in vitro

Research output: Contribution to journal › Article › peer-review

Abstract

A simple procedure for the quantitative isolation of small amounts of newly synthesized radioactive nucleic acids is described which uses a CF-11 cellulose matrix. More than 80% of the DNA product synthesized in an endogenous reaction using detergent-disrupted Rauscher leukemia virus or in a purified system consisting of viral RNA and avian myeloblastosis virus reverse transcriptase can be recovered free of contaminating, labeled RNA precursors and in the absence of carrier.

Original language	English (US)
Pages (from-to)	340-344
Number of pages	5
Journal	Analytical Biochemistry
Volume	75
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(76)90087-7
State	Published - Sep 1976
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(76)90087-7

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title = "A simplified procedure for the quantitative isolation of DNA and RNA transcripts synthesized in vitro",

abstract = "A simple procedure for the quantitative isolation of small amounts of newly synthesized radioactive nucleic acids is described which uses a CF-11 cellulose matrix. More than 80% of the DNA product synthesized in an endogenous reaction using detergent-disrupted Rauscher leukemia virus or in a purified system consisting of viral RNA and avian myeloblastosis virus reverse transcriptase can be recovered free of contaminating, labeled RNA precursors and in the absence of carrier.",

author = "Modak, {M. J.}",

note = "Funding Information: This research is supported by National Cancer Institute Grant CA 08748. I thank Dr. S. Marcus and S. Smith for stimulating discussion and Drs. D. Jagannath and W. Prensky for some of the materials.",

year = "1976",

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doi = "10.1016/0003-2697(76)90087-7",

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PY - 1976/9

Y1 - 1976/9

N2 - A simple procedure for the quantitative isolation of small amounts of newly synthesized radioactive nucleic acids is described which uses a CF-11 cellulose matrix. More than 80% of the DNA product synthesized in an endogenous reaction using detergent-disrupted Rauscher leukemia virus or in a purified system consisting of viral RNA and avian myeloblastosis virus reverse transcriptase can be recovered free of contaminating, labeled RNA precursors and in the absence of carrier.

AB - A simple procedure for the quantitative isolation of small amounts of newly synthesized radioactive nucleic acids is described which uses a CF-11 cellulose matrix. More than 80% of the DNA product synthesized in an endogenous reaction using detergent-disrupted Rauscher leukemia virus or in a purified system consisting of viral RNA and avian myeloblastosis virus reverse transcriptase can be recovered free of contaminating, labeled RNA precursors and in the absence of carrier.

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JO - Analytical Biochemistry

JF - Analytical Biochemistry

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ER -

A simplified procedure for the quantitative isolation of DNA and RNA transcripts synthesized in vitro

Abstract

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