A TAG1-APP signalling pathway through Fe65 negatively modulates neurogenesis

Quan Hong Ma, Toshitaka Futagawa, Wu Lin Yang, Xiao Dan Jiang, Li Zeng, Yasuo Takeda, Ru Xiang Xu, Dominique Bagnard, Melitta Schachner, Andrew J. Furley, Domna Karagogeos, Kazutada Watanabe, Gavin S. Dawe, Zhi Cheng Xiao

Research output: Contribution to journalArticlepeer-review

160 Scopus citations


The release of amyloid precursor protein (APP) intracellular domain (AICD) may be triggered by extracellular cues through γ-secretase-dependent cleavage. AICD binds to Fe65, which may have a role in AICD-dependent signalling; however, the functional ligand has not been characterized. In this study, we have identified TAG1 as a functional ligand of APP. We found that, through an extracellular interaction with APP, TAG1 increased AICD release and triggered Fe65-dependent activity in a γ-secretase-dependent manner. TAG1, APP and Fe65 colocalized in the neural stem cell niche of the fetal ventricular zone. Neural precursor cells from TAG1-/-, APP-/- and TAG1-/-; APP-/- mice had aberrantly enhanced neurogenesis, which was significantly reversed in TAG1-/- mice by TAG1 or AICD but not by AICD mutated at the Fe65 binding site. Notably, TAG1 reduced normal neurogenesis in Fe65+/+ mice. Abnormally enhanced neurogenesis also occurred in Fe65-/- mice but could not be reversed by TAG1. These results describe a TAG1-APP signalling pathway that negatively modulates neurogenesis through Fe65.

Original languageEnglish (US)
Pages (from-to)283-294
Number of pages12
JournalNature Cell Biology
Issue number3
StatePublished - Mar 2008

All Science Journal Classification (ASJC) codes

  • Cell Biology


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