A toxic shock syndrome toxin mutant of Staphylococcus aureus isolated by allelic replacement lacks virulence in a rabbit uterine model

Rhona Sloane; Joyce C.S. de Azavedo; John P. Arbuthnott; Patrick J. Hartigan; Barry Kreiswirth; Richard Novick; Timothy J. Foster

doi:10.1111/j.1574-6968.1991.tb04449.x

A toxic shock syndrome toxin mutant of Staphylococcus aureus isolated by allelic replacement lacks virulence in a rabbit uterine model

Rhona Sloane, Joyce C.S. de Azavedo, John P. Arbuthnott, Patrick J. Hartigan, Barry Kreiswirth, Richard Novick, Timothy J. Foster

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The gene coding for toxic shock syndrome toxin-1 in S. aureus was inactivated by allelic replacement in two TSS-associated strains. One mutant derived from FRI1169 (a non-enterotoxigenic strain) lacked virulence in the rabbit uterine chamber infection model. This suggests that TSST-1 is the only determinant produced by this strain that can induce the symptoms of shock in rabbits. A novel method for allelic replacement involving transduction of plasmid integrants is described.

Original language	English (US)
Pages (from-to)	239-244
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	78
Issue number	2-3
DOIs	https://doi.org/10.1111/j.1574-6968.1991.tb04449.x
State	Published - Mar 1 1991
Externally published	Yes

All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology
Genetics

Keywords

Allele replacement mutant
Animal model
Staphylococcus aureus
Toxic shock syndrome toxin

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10.1111/j.1574-6968.1991.tb04449.x

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title = "A toxic shock syndrome toxin mutant of Staphylococcus aureus isolated by allelic replacement lacks virulence in a rabbit uterine model",

abstract = "The gene coding for toxic shock syndrome toxin-1 in S. aureus was inactivated by allelic replacement in two TSS-associated strains. One mutant derived from FRI1169 (a non-enterotoxigenic strain) lacked virulence in the rabbit uterine chamber infection model. This suggests that TSST-1 is the only determinant produced by this strain that can induce the symptoms of shock in rabbits. A novel method for allelic replacement involving transduction of plasmid integrants is described.",

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AU - Sloane, Rhona

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AU - Arbuthnott, John P.

AU - Hartigan, Patrick J.

AU - Kreiswirth, Barry

AU - Novick, Richard

AU - Foster, Timothy J.

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AB - The gene coding for toxic shock syndrome toxin-1 in S. aureus was inactivated by allelic replacement in two TSS-associated strains. One mutant derived from FRI1169 (a non-enterotoxigenic strain) lacked virulence in the rabbit uterine chamber infection model. This suggests that TSST-1 is the only determinant produced by this strain that can induce the symptoms of shock in rabbits. A novel method for allelic replacement involving transduction of plasmid integrants is described.

KW - Allele replacement mutant

KW - Animal model

KW - Staphylococcus aureus

KW - Toxic shock syndrome toxin

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A toxic shock syndrome toxin mutant of Staphylococcus aureus isolated by allelic replacement lacks virulence in a rabbit uterine model

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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