TY - JOUR
T1 - A unified DNA- and dNTP-binding mode for DNA polymerases
AU - Singh, Kamalendra
AU - Modak, Mukund J.
N1 - Funding Information:
This work was supported by a research grant from the NIH (NIH grant GM36307). We thank Charles Richardson and colleagues for sharing results on the T7 DNA polymerase ternary complex prior to publication. We also thank Stefan Sarafianos for critically reading the manuscript.
PY - 1998/8/1
Y1 - 1998/8/1
N2 - Crystal structures of various DNA polymerases show a common structural topology that resembles a right hand and has distinct finger, palm and thumb subdomains. Early models of the Klenow fragment (KF) of Escherichia coli polymerase I showed DNA entering a large cleft that faces the palm subdomain where the catalytic site is situated. However, subsequent resolution of the structures of HIV-1 reverse transcriptase, KF and polymerase β (pol β) bound to DNA yielded conflicting data that suggested a different orientation for DNA bound to pol β compared with DNA bound to other polymerases. The debate, on the correct orientation of the template-primer DNA, that followed failed to reach a consensus. Using an alternative superposition scheme, we now provide convincing evidence for a common DNA-binding mode that is applicable to all polymerases, including pol β. Copyright (C) 1998 Elsevier Science Ltd.
AB - Crystal structures of various DNA polymerases show a common structural topology that resembles a right hand and has distinct finger, palm and thumb subdomains. Early models of the Klenow fragment (KF) of Escherichia coli polymerase I showed DNA entering a large cleft that faces the palm subdomain where the catalytic site is situated. However, subsequent resolution of the structures of HIV-1 reverse transcriptase, KF and polymerase β (pol β) bound to DNA yielded conflicting data that suggested a different orientation for DNA bound to pol β compared with DNA bound to other polymerases. The debate, on the correct orientation of the template-primer DNA, that followed failed to reach a consensus. Using an alternative superposition scheme, we now provide convincing evidence for a common DNA-binding mode that is applicable to all polymerases, including pol β. Copyright (C) 1998 Elsevier Science Ltd.
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U2 - 10.1016/S0968-0004(98)01250-X
DO - 10.1016/S0968-0004(98)01250-X
M3 - Article
C2 - 9757823
AN - SCOPUS:0031680053
SN - 0968-0004
VL - 23
SP - 277
EP - 281
JO - Trends in Biochemical Sciences
JF - Trends in Biochemical Sciences
IS - 8
ER -