@article{6cc37af60f52429db6ccefb1ca9159c1,
title = "A universal allyl linker for solid-phase synthesis",
abstract = "We report synthesis of a universal allyl linker for solid-phase synthesis, 9-O-(4,4'-Dimethoxytrityl)-10-undecenoic (3), that has a reactive terminal double bond. Since allyl cleavage occurs under conditions orthogonal to those used during the solid-phase synthesis and deprotection of DNA or RNA fragments, this linker extends the range of post-synthetic manipulations that can be carried out without cleavage from the support, and means that this linkage could be used to construct affinity columns. Alternatively, it should be possible also to cleave fully protected molecules from the support if so desired.",
author = "Xiaohu Zhang and Jones, {Roger A.}",
note = "Funding Information: Automated DNA or RNA synthesis with support-bound 3 can be carried out by any of the standard nucleic acid synthetic methods. Importantly, for RNA synthesis desilylation with TBAF or TEAo3HF can be carried out while the RNA fragment is attached to the support, and the excess reagent removed simply by washing the support-bound RNA with appropriate solvents. In addition, the ammonia treatment will cleave DNA fragments at any depurinated sites so that the 5'-DMT portion of these fragments is removed, thereby significantly simplifying the purification. 16 Cleavage of the nucleic acid fragment from the support can be effected under the conditions reported by Noyori 8 to give the oligomer as its 3'-phosphate. 17 Acknow~dgment. This work was supported by a grant ~om the NationalInstitutes of Health (GM48802).",
year = "1996",
month = may,
doi = "10.1016/0040-4039(96)00714-9",
language = "English (US)",
volume = "37",
pages = "3789--3790",
journal = "Tetrahedron Letters",
issn = "0040-4039",
publisher = "Elsevier Limited",
number = "22",
}