A yeast acetyl coenzyme A carboxylase mutant links very-long-chain fatty acid synthesis to the structure and function of the nuclear membrane-pore complex

Roger Schneiter, Midori Hitomi, Andreas Ivessa, Evelyn Verena Fasch, Sepp D. Kohlwein, Alan M. Tartakoff

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

The conditional mRNA transport mutant of Saccharomyces cerevisiae, acc1- 7-1 (mtr7-1), displays a unique alteration of the nuclear envelope. Unlike nucleoporin mutants and other RNA transport mutants, the intermembrane space expands, protuberances extend from the inner membrane into the intermembrane space, and vesicles accumulate in the intermembrane space. MTR7 is the same gene as ACC1, encoding acetyl coenzyme A (CoA) carboxylase (Acc1p), the rate- limiting enzyme of de novo fatty acid synthesis. Genetic and biochemical analyses of fatty acid synthesis mutants and acc1-7-1 indicate that the continued synthesis of malonyl-CoA, the enzymatic product of acetyl-CoA carboxylase, is required for an essential pathway which is independent from de novo synthesis of fatty acids. We provide evidence that synthesis of very- long-chain fatty acids (C26 atoms) is inhibited in acc1-7-1, suggesting that very-long-chain fatty acid synthesis is required to maintain a functional nuclear envelope.

Original languageEnglish (US)
Pages (from-to)7161-7172
Number of pages12
JournalMolecular and cellular biology
Volume16
Issue number12
DOIs
StatePublished - Jan 1 1996

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Acetyl-CoA Carboxylase
Nuclear Pore
Nuclear Envelope
Fatty Acids
Yeasts
RNA Transport
Nuclear Pore Complex Proteins
Malonyl Coenzyme A
Saccharomyces cerevisiae
Molecular Biology
Messenger RNA
Membranes
Enzymes
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Schneiter, Roger ; Hitomi, Midori ; Ivessa, Andreas ; Fasch, Evelyn Verena ; Kohlwein, Sepp D. ; Tartakoff, Alan M. / A yeast acetyl coenzyme A carboxylase mutant links very-long-chain fatty acid synthesis to the structure and function of the nuclear membrane-pore complex. In: Molecular and cellular biology. 1996 ; Vol. 16, No. 12. pp. 7161-7172.
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A yeast acetyl coenzyme A carboxylase mutant links very-long-chain fatty acid synthesis to the structure and function of the nuclear membrane-pore complex. / Schneiter, Roger; Hitomi, Midori; Ivessa, Andreas; Fasch, Evelyn Verena; Kohlwein, Sepp D.; Tartakoff, Alan M.

In: Molecular and cellular biology, Vol. 16, No. 12, 01.01.1996, p. 7161-7172.

Research output: Contribution to journalArticle

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