ABBA regulates plasma-membrane and actin dynamics to promote radial glia extension

Juha Saarikangas, Janne Hakanen, Pieta K. Mattila, Martin Grumet, Marjo Salminen, Pekka Lappalainen

Research output: Contribution to journalArticlepeer-review

40 Scopus citations


Radial glia play key inal migration, axon guidance, and neurogenesis during development of the central nervous system. However, the molecular mechanisms regulating growth and morphology of these extended cells are unknown. We show that ABBA, a novel member of the IRSp53-MIM protein family, is enriched in different types of radial glia. ABBA binds ATP-actin monomers with high affinity and deforms PtdIns(4,5)P2-rich membranes in vitro through its WH2 and IM domains, respectively. In radial-glia-like C6-R cells, ABBA localises to the interface between the actin cytoskeleton and plasma membrane, and its depletion by RNAi led to defects in lamellipodial dynamics and process extension. Together, this study identifies ABBA as a novel regulator of actin and plasma membrane dynamics in radial glial cells, and provides evidence that membrane binding and deformation activity is critical for the cellular functions of IRSp53-MIM-ABBA family proteins.

Original languageEnglish (US)
Pages (from-to)1444-1454
Number of pages11
JournalJournal of cell science
Issue number9
StatePublished - May 1 2008

All Science Journal Classification (ASJC) codes

  • Cell Biology


  • ABBA
  • Actin
  • Cytoskeleton
  • IMD
  • Membrane deformation
  • Radial glia


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