TY - JOUR
T1 - Aberrant cortical activity in multiple GCaMP6-expressing transgenic mouse lines
AU - Steinmetz, Nicholas A.
AU - Buetfering, Christina
AU - Lecoq, Jerome
AU - Lee, Christian R.
AU - Peters, Andrew J.
AU - Jacobs, Elina A.K.
AU - Coen, Philip
AU - Ollerenshaw, Douglas R.
AU - Valley, Matthew T.
AU - De Vries, Saskia E.J.
AU - Garrett, Marina
AU - Zhuang, Jun
AU - Groblewski, Peter A.
AU - Manavi, Sahar
AU - Miles, Jesse
AU - White, Casey
AU - Lee, Eric
AU - Griffin, Fiona
AU - Larkin, Joshua D.
AU - Roll, Kate
AU - Cross, Sissy
AU - Nguyen, Thuyanh V.
AU - Larsen, Rachael
AU - Pendergraft, Julie
AU - Daigle, Tanya
AU - Tasic, Bosiljka
AU - Thompson, Carol L.
AU - Waters, Jack
AU - Olsen, Shawn
AU - Margolis, David J.
AU - Zeng, Hongkui
AU - Hausser, Michael
AU - Carandini, Matteo
AU - Harris, Kenneth D.
N1 - Funding Information:
EC | Horizon 2020 (EU Framework Programme for Research and Innovation); Human Frontier Science Program (HFSP); European Molecular Biology Organization (EMBO); Wellcome; Biotechnology and Biological Sciences Research Council (BBSRC); EC | European Research Council (ERC); New Jersey Commission on Brain Injury Research (NJCBIR); National Science Foundation (NSF); HHS | National Institutes of Health (NIH). N.A.S. was supported by postdoctoral fellowships from the HFSP and the Marie Curie Action of the EU. C.B. was supported by an EMBO Long-Term Fellowship and the Marie Curie Action of the EU. M.H. was supported by the Wellcome Trust, BBSRC, and ERC. D.J.M. was supported by the NJCBIR, the NSF, and NIH. M.C. holds the GlaxoSmithKline / Fight for Sight Chair in Visual Neuroscience.
Funding Information:
EC | Horizon 2020 (EU Framework Programme for Research and Innovation); Human Frontier Science Program (HFSP); European Molecular Biology Organization (EMBO); Wellcome; Biotechnology and Biological Sciences Research Council (BBSRC); EC | European Research Council (ERC); New Jersey Commission on Brain Injury Research (NJCBIR); National Science Foundation (NSF); HHS | National Institutes of Health (NIH). N.A.S. was supported by postdoctoral fellowships from the HFSP and the Marie Curie Action of the EU. C.B. was supported by an EMBO Long-Term Fellowship and the Marie Curie Action of the EU. M.H. was supported by the Wellcome Trust, BBSRC, and ERC. D.J.M. was supported by the NJCBIR, the NSF, and NIH. M.C. holds the GlaxoSmithKline/ Fight for Sight Chair in Visual Neuroscience. Acknowledgments: We thank Federico Rossi, Efthymia Diamanti, Sam Failor, Adam Packer, Mehmet Fisek, and Spencer Smith for helpful discussions. We thank Charu Reddy and Soyon Chun for help with animal husbandry.
Publisher Copyright:
© 2017 Steinmetz et al.
PY - 2017/9/1
Y1 - 2017/9/1
N2 - Transgenic mouse lines are invaluable tools for neuroscience but, as with any technique, care must be taken to ensure that the tool itself does not unduly affect the system under study. Here we report aberrant electrical activity, similar to interictal spikes, and accompanying fluorescence events in some genotypes of transgenic mice expressing GCaMP6 genetically encoded calcium sensors. These epileptiform events have been observed particularly, but not exclusively, in mice with Emx1-Cre and Ai93 transgenes, of either sex, across multiple laboratories. The events occur at >0.1 Hz, are very large in amplitude (>1.0 mV local field potentials, >10% df/f widefield imaging signals), and typically cover large regions of cortex. Many properties of neuronal responses and behavior seem normal despite these events, although rare subjects exhibit overt generalized seizures. The underlying mechanisms of this phenomenon remain unclear, but we speculate about possible causes on the basis of diverse observations. We encourage researchers to be aware of these activity patterns while interpreting neuronal recordings from affected mouse lines and when considering which lines to study.
AB - Transgenic mouse lines are invaluable tools for neuroscience but, as with any technique, care must be taken to ensure that the tool itself does not unduly affect the system under study. Here we report aberrant electrical activity, similar to interictal spikes, and accompanying fluorescence events in some genotypes of transgenic mice expressing GCaMP6 genetically encoded calcium sensors. These epileptiform events have been observed particularly, but not exclusively, in mice with Emx1-Cre and Ai93 transgenes, of either sex, across multiple laboratories. The events occur at >0.1 Hz, are very large in amplitude (>1.0 mV local field potentials, >10% df/f widefield imaging signals), and typically cover large regions of cortex. Many properties of neuronal responses and behavior seem normal despite these events, although rare subjects exhibit overt generalized seizures. The underlying mechanisms of this phenomenon remain unclear, but we speculate about possible causes on the basis of diverse observations. We encourage researchers to be aware of these activity patterns while interpreting neuronal recordings from affected mouse lines and when considering which lines to study.
KW - Cortex
KW - Epilepsy
KW - GCaMP
KW - Transgenic
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UR - http://www.scopus.com/inward/citedby.url?scp=85032150788&partnerID=8YFLogxK
U2 - 10.1523/ENEURO.0207-17.2017
DO - 10.1523/ENEURO.0207-17.2017
M3 - Article
C2 - 28932809
AN - SCOPUS:85032150788
SN - 2373-2822
VL - 4
JO - eNeuro
JF - eNeuro
IS - 5
M1 - e0207-17.2017
ER -