Abortive initiation and productive initiation by RNA polymerase involve DNA scrunching

Andrey Revyakin, Chenyu Liu, Richard H. Ebright, Terence R. Strick

Research output: Contribution to journalArticlepeer-review

279 Scopus citations

Abstract

Using single-molecule DNA nanomanipulation, we show that abortive initiation involves DNA "scrunching"-in which RNA polymerase (RNAP) remains stationary and unwinds and pulls downstream DNA into itself-and that scrunching requires RNA synthesis and depends on RNA length. We show further that promoter escape involves scrunching, and that scrunching occurs in most or all instances of promoter escape. Our results support the existence of an obligatory stressed intermediate, with approximately one turn of additional DNA unwinding, in escape and are consistent with the proposal that stress in this intermediate provides the driving force to break RNAP-promoter and RNAP-initiation-factor interactions in escape.

Original languageEnglish (US)
Pages (from-to)1139-1143
Number of pages5
JournalScience
Volume314
Issue number5802
DOIs
StatePublished - Nov 17 2006

All Science Journal Classification (ASJC) codes

  • General

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