Acetyl coenzyme A synthetase catalyzed reactions of coenzyme A with α,β-unsaturated carboxylic acids

Smita S. Patel, David R. Walt

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

α,β-Unsaturated coenzyme A (CoA) thioesters including acrylyl CoA, methacrylyl CoA, and propiolyl CoA were synthesized by catalysis with acetyl CoA synthetase (EC 6.2.1.1). After isolation from the enzymatic reactions, the products were found to be the result of 1,4 addition of CoASH to the double bond and addition of water to the triple bond of the initial acyl CoA adducts. Structural determinations of these products by 1H NMR, 13C NMR, and the chemical reactions leading to their formation are described.

Original languageEnglish (US)
Pages (from-to)355-360
Number of pages6
JournalAnalytical Biochemistry
Volume170
Issue number2
DOIs
StatePublished - May 1 1988
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Keywords

  • C-13 NMR
  • acetyl CoA synthetase
  • acrylyl CoA
  • methacrylyl CoA
  • propiolyl CoA
  • proton NMR

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