Acid soluble nuclear proteins of rat liver: Differential absorbance of bound dyes and changes in neoplasia

M. A. Lea; L. A. Youngworth; H. P. Morris

doi:10.1016/S0006-291X(74)80496-1

Acid soluble nuclear proteins of rat liver: Differential absorbance of bound dyes and changes in neoplasia

M. A. Lea, L. A. Youngworth, H. P. Morris

New Jersey Medical School (NJMS), Biochemistry

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Evidence was obtained for the presence in several hepatomas of a minor histone previously reported to be absent in tumors. The concentration of this protein was inversely related to hepatoma growth rate. Comparison of light absorbance by bound dyes showed that the protein appeared to be a minor component of acid soluble nuclear proteins when stained with Amido Black or Fast Green but a major component when stained with Coomassie blue.

Original language	English (US)
Pages (from-to)	862-867
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	58
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(74)80496-1
State	Published - Jun 4 1974

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(74)80496-1

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title = "Acid soluble nuclear proteins of rat liver: Differential absorbance of bound dyes and changes in neoplasia",

abstract = "Evidence was obtained for the presence in several hepatomas of a minor histone previously reported to be absent in tumors. The concentration of this protein was inversely related to hepatoma growth rate. Comparison of light absorbance by bound dyes showed that the protein appeared to be a minor component of acid soluble nuclear proteins when stained with Amido Black or Fast Green but a major component when stained with Coomassie blue.",

author = "Lea, {M. A.} and Youngworth, {L. A.} and Morris, {H. P.}",

note = "Funding Information: Acknowledgements. This work was supported in part by USPHS Grants CA-I0729 and CA-12933. We are grateful to Ms. C. M. Jackson and Ms. Louise Lawson for technical assistance and to Mr. R. A. Daly and Mr. D. Monahan for their participation in preliminary studies.",

year = "1974",

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doi = "10.1016/S0006-291X(74)80496-1",

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T1 - Acid soluble nuclear proteins of rat liver

T2 - Differential absorbance of bound dyes and changes in neoplasia

AU - Lea, M. A.

AU - Youngworth, L. A.

AU - Morris, H. P.

N1 - Funding Information: Acknowledgements. This work was supported in part by USPHS Grants CA-I0729 and CA-12933. We are grateful to Ms. C. M. Jackson and Ms. Louise Lawson for technical assistance and to Mr. R. A. Daly and Mr. D. Monahan for their participation in preliminary studies.

PY - 1974/6/4

Y1 - 1974/6/4

N2 - Evidence was obtained for the presence in several hepatomas of a minor histone previously reported to be absent in tumors. The concentration of this protein was inversely related to hepatoma growth rate. Comparison of light absorbance by bound dyes showed that the protein appeared to be a minor component of acid soluble nuclear proteins when stained with Amido Black or Fast Green but a major component when stained with Coomassie blue.

AB - Evidence was obtained for the presence in several hepatomas of a minor histone previously reported to be absent in tumors. The concentration of this protein was inversely related to hepatoma growth rate. Comparison of light absorbance by bound dyes showed that the protein appeared to be a minor component of acid soluble nuclear proteins when stained with Amido Black or Fast Green but a major component when stained with Coomassie blue.

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IS - 3

ER -

Acid soluble nuclear proteins of rat liver: Differential absorbance of bound dyes and changes in neoplasia

Abstract

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