The tumor-promoting activities of multiple applications of phorbol and of four phorbol diesters as well as the effect of a single application of each on the incorporation of cytidine-3H into skin RNA were determined in female Charles River CD1 mice. Effective tumor formation correlated with a stimulation of cytidine-3H incorporation. The nature of the early RNA response was analyzed by polyacrylamide gel electrophoresis. The extraction procedure allowed quantitative isolation of undegraded RNA from whole mouse skin. By 3 hr after treatment with effective tumor promoters, there was a large stimulation of incorporation of cytidine-3H into “32 S” RNA, rRNA, and 4 to 5 S RNA. Stimulation of cytidine-3H incorporation into 32 S RNA was observed as early as 30 min after treatment with the most effective promoter, 12-0-tetradecanoyl-phorbol-13-acetate. Comparison of the responses in cytidine-3 H incorporation 3 hr after treatment with tumor-promoting phorbol esters and 2 irritants suggested that the phorbol esters were able to induce increased incorporation into 32 S RNA earlier than the irritants, while both caused increased incorporation into the 4 to 5 S RNA.
|Original language||English (US)|
|Number of pages||8|
|State||Published - Apr 1972|
All Science Journal Classification (ASJC) codes
- Cancer Research