Acrylamide Gel Electrophoresis Studies of the Incorporation of Cytidine-3H into Mouse Skin RNA at Early Times after Treatment with Phorbol Esters

William M. Baird, Peter W. Melera, R. K. Boutwell

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30 Scopus citations

Abstract

The tumor-promoting activities of multiple applications of phorbol and of four phorbol diesters as well as the effect of a single application of each on the incorporation of cytidine-3H into skin RNA were determined in female Charles River CD1 mice. Effective tumor formation correlated with a stimulation of cytidine-3H incorporation. The nature of the early RNA response was analyzed by polyacrylamide gel electrophoresis. The extraction procedure allowed quantitative isolation of undegraded RNA from whole mouse skin. By 3 hr after treatment with effective tumor promoters, there was a large stimulation of incorporation of cytidine-3H into “32 S” RNA, rRNA, and 4 to 5 S RNA. Stimulation of cytidine-3H incorporation into 32 S RNA was observed as early as 30 min after treatment with the most effective promoter, 12-0-tetradecanoyl-phorbol-13-acetate. Comparison of the responses in cytidine-3 H incorporation 3 hr after treatment with tumor-promoting phorbol esters and 2 irritants suggested that the phorbol esters were able to induce increased incorporation into 32 S RNA earlier than the irritants, while both caused increased incorporation into the 4 to 5 S RNA.

Original languageEnglish (US)
Pages (from-to)781-788
Number of pages8
JournalCancer Research
Volume32
Issue number4
StatePublished - Apr 1972

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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