Acting on actin: The electric motility assay

Daniel Riveline; Albrecht Ott; Frank Jülicher; Donald A. Winkelmann; Olivier Cardoso; Jean Jacques Lacapère; Soffia Magnúsdóttir; Jean Louis Viovy; Laurence Gorre-Talini; Jacques Prost

doi:10.1007/s002490050147

Acting on actin: The electric motility assay

Daniel Riveline, Albrecht Ott, Frank Jülicher, Donald A. Winkelmann, Olivier Cardoso, Jean Jacques Lacapère, Soffia Magnúsdóttir, Jean Louis Viovy, Laurence Gorre-Talini, Jacques Prost

Robert Wood Johnson Medical School (RWJMS), Pathology

Research output: Contribution to journal › Article › peer-review

136 Scopus citations

Abstract

We have developed a novel technique which allows one to direct the two dimensional motion of actin filaments on a myosin coated sheet using a weak electric field parallel to the plane of motion. The filament velocity can be increased or decreased, and even reversed, as a function of orientation and strength of the field. PMMA (poly(methylmethacrylate)) gratings, which act as rails for actin, allow one for the first time to explore three quadrants of the force velocity diagram. We discuss effective friction, duty ratio and stall force at different myosin densities. A discontinuity in the velocity force relationship suggests the existence of dynamical phase transition.

Original language	English (US)
Pages (from-to)	403-408
Number of pages	6
Journal	European Biophysics Journal
Volume	27
Issue number	4
DOIs	https://doi.org/10.1007/s002490050147
State	Published - 1998

All Science Journal Classification (ASJC) codes

Biophysics

Keywords

Actin
Electric fields
Instability
Myosin
PMMA

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10.1007/s002490050147

Cite this

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title = "Acting on actin: The electric motility assay",

abstract = "We have developed a novel technique which allows one to direct the two dimensional motion of actin filaments on a myosin coated sheet using a weak electric field parallel to the plane of motion. The filament velocity can be increased or decreased, and even reversed, as a function of orientation and strength of the field. PMMA (poly(methylmethacrylate)) gratings, which act as rails for actin, allow one for the first time to explore three quadrants of the force velocity diagram. We discuss effective friction, duty ratio and stall force at different myosin densities. A discontinuity in the velocity force relationship suggests the existence of dynamical phase transition.",

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T1 - Acting on actin

T2 - The electric motility assay

AU - Riveline, Daniel

AU - Ott, Albrecht

AU - Jülicher, Frank

AU - Winkelmann, Donald A.

AU - Cardoso, Olivier

AU - Lacapère, Jean Jacques

AU - Magnúsdóttir, Soffia

AU - Viovy, Jean Louis

AU - Gorre-Talini, Laurence

AU - Prost, Jacques

PY - 1998

Y1 - 1998

N2 - We have developed a novel technique which allows one to direct the two dimensional motion of actin filaments on a myosin coated sheet using a weak electric field parallel to the plane of motion. The filament velocity can be increased or decreased, and even reversed, as a function of orientation and strength of the field. PMMA (poly(methylmethacrylate)) gratings, which act as rails for actin, allow one for the first time to explore three quadrants of the force velocity diagram. We discuss effective friction, duty ratio and stall force at different myosin densities. A discontinuity in the velocity force relationship suggests the existence of dynamical phase transition.

AB - We have developed a novel technique which allows one to direct the two dimensional motion of actin filaments on a myosin coated sheet using a weak electric field parallel to the plane of motion. The filament velocity can be increased or decreased, and even reversed, as a function of orientation and strength of the field. PMMA (poly(methylmethacrylate)) gratings, which act as rails for actin, allow one for the first time to explore three quadrants of the force velocity diagram. We discuss effective friction, duty ratio and stall force at different myosin densities. A discontinuity in the velocity force relationship suggests the existence of dynamical phase transition.

KW - Actin

KW - Electric fields

KW - Instability

KW - Myosin

KW - PMMA

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JO - European Biophysics Journal

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Acting on actin: The electric motility assay

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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